ArticleApplied and environmental microbiology2004
Use of merocyanine 540 for photodynamic inactivation of Staphylococcus aureus planktonic and biofilm cells.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Photodynamic Inactivation Mediated by Endogenous Porphyrins ofACS omega · 2025Article
- Phototherapy and optical waveguides for the treatment of infection.Advanced drug delivery reviews · 2021Review
- Potential of Cyanine Derived Dyes in Photodynamic Therapy.Pharmaceutics · 2021Review
- The efficacy of photodynamic inactivation with laser diode onInfectious disease reports · 2020Article
- Light based anti-infectives: ultraviolet C irradiation, photodynamic therapy, blue light, and beyond.Current opinion in pharmacology · 2013Review
- Photodynamic inactivation of biofilm: taking a lightly colored approach to stubborn infection.Expert review of anti-infective therapy · 2013Review
- Effect of Sodium Chloride on Aggregation of Merocyanine 540 and Photosensitized Inactivation of Staphylococcus aureus and Pseudomonas aeruginosa.Acta naturae · 2011Article
- Optical method for monitoring of photodynamic inactivation of bacteria.Journal of biological physics · 2011Article
- The use of drip flow and rotating disk reactors for Staphylococcus aureus biofilm analysis.Journal of visualized experiments : JoVE · 2010Article
- Light sources for photodynamic inactivation of bacteria.Lasers in medical science · 2009Review
- Toluidine blue-mediated photodynamic effects on staphylococcal biofilms.Antimicrobial agents and chemotherapy · 2008Article
- Article
- Selective photonic disinfection of cell culture using a visible ultrashort pulsed laser.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics SocietyArticle
- Review
- Strategies to potentiate antimicrobial photoinactivation by overcoming resistant phenotypes.Photochemistry and photobiologyReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photodynamic inactivation of Staphylococcus aureus planktonic and biofilm cells by a phtotosensitizer, merocyanine 540 (MC 540), was investigated. For the planktonic experiments, MC 540 binding efficiency to bacterial cells was found to increase with both increasing MC 540 concentration and increasing incubation time, but the binding became saturated following 10 min of incubation. The antimicrobial activity was enhanced with an increasing light dose, but an increase in the light dose could not further improve the antimicrobial activity if the maximum excitation level attainable was less than the necessary minimum threshold level. Complete inactivation was achieved when the excitation level of MC 540 was somewhere above the threshold level. The relationship between antimicrobial activity and the excitation level of MC 540 revealed that the more MC 540 was excited, the more S. aureus cells were killed. For the biofilm experiments, the antimicrobial activity was enhanced with an increase in the light dose. No viable cells were detected when organisms were exposed to 15 mug of MC 540 per ml and a light dose of 600 J/cm2 or to 20 mug of MC 540 per ml and a light dose of 450 J/cm2. A quantitative analysis of MC 540 bound to biofilms was also performed, and the images from confocal laser scanning microscopy provided direct evidence that revealed the difference between the MC 540 remaining in the biofilms prior to irradiation and the MC 540 remaining in the biofilms after irradiation. The results of both the planktonic and biofilm experiments suggest that the antimicrobial activity of photodynamic inactivation of S. aureus is closely related to the excitation level of MC 540.
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