ArticleApplied and environmental microbiology2004
Highly efficient production of laccase by the basidiomycete Pycnoporus cinnabarinus.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 106 citations in OpenAlex.
- Eco-Powered Cleanup: Laccase as a Green Catalyst for Tackling Emerging Contaminants.Global challenges (Hoboken, NJ) · 2025Review
- Sustainable, Targeted, and Cost-Effective Laccase-Based Bioremediation Technologies for Antibiotic Residues in the Ecosystem: A Comprehensive Review.Biomolecules · 2025Review
- Towards engineering agaricomycete fungi for terpenoid production.Journal of industrial microbiology & biotechnology · 2024Review
- Production, purification, and characterization of p-diphenol oxidase (PDO) enzyme from lignolytic fungal isolate Schizophyllum commune MF-O5.Folia microbiologica · 2023Article
- Fungal Laccases: Fundamentals, Engineering and Classification Update.Biomolecules · 2023Review
- H3K4me2 ChIP-Seq reveals the epigenetic landscape during mushroom formation and novel developmental regulators of Schizophyllum commune.Scientific reports · 2021Article
- High-throughput targeted gene deletion in the model mushroom Schizophyllum commune using pre-assembled Cas9 ribonucleoproteins.Scientific reports · 2019Article
- Signal peptide replacement resulted in recombinant homologous expression of laccase Lcc8 in Coprinopsis cinerea.AMB Express · 2019Article
- Integrative visual omics of the white-rot fungusBiotechnology for biofuels · 2018Article
- Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation.Frontiers in microbiology · 2017Review
- Yeast Hosts for the Production of Recombinant Laccases: A Review.Molecular biotechnology · 2016Review
- High Level Secretion of Laccase (LccH) from a Newly Isolated White-Rot Basidiomycete, Hexagonia hirta MSF2.Frontiers in microbiology · 2016Article
- Cloning and Expression Analysis of Vvlcc3, a Novel and Functional Laccase Gene Possibly Involved in Stipe Elongation.International journal of molecular sciences · 2015Article
- Transcriptional profiles of laccase genes in the brown rot fungus Postia placenta MAD-R-698.Journal of microbiology (Seoul, Korea) · 2015Article
- Identification and characterization of laccase-type multicopper oxidases involved in dye-decolorization by the fungus Leptosphaerulina sp.BMC biotechnology · 2015Article
- The genome of the white-rot fungus Pycnoporus cinnabarinus: a basidiomycete model with a versatile arsenal for lignocellulosic biomass breakdown.BMC genomics · 2014Article
- Enhancement of ganoderic acid accumulation by overexpression of an N-terminally truncated 3-hydroxy-3-methylglutaryl coenzyme A reductase gene in the basidiomycete Ganoderma lucidum.Applied and environmental microbiology · 2012Article
- Engineering platforms for directed evolution of Laccase from Pycnoporus cinnabarinus.Applied and environmental microbiology · 2012Article
- Multiple multi-copper oxidase gene families in basidiomycetes - what for?Current genomics · 2011Article
- Improved Laccase Production by Trametes pubescens MB89 in Distillery Wastewaters.Enzyme research · 2011Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An efficient transformation and expression system was developed for the industrially relevant basidiomycete Pycnoporus cinnabarinus. This was used to transform a laccase-deficient monokaryotic strain with the homologous lac1 laccase gene placed under the regulation of its own promoter or that of the SC3 hydrophobin gene or the glyceraldehyde-3-phosphate dehydrogenase (GPD) gene of Schizophyllum commune. SC3-driven expression resulted in a maximal laccase activity of 107 nkat ml(-1) in liquid shaken cultures. This value was about 1.4 and 1.6 times higher in the cases of the GPD and lac1 promoters, respectively. lac1-driven expression strongly increased when 25 g of ethanol liter(-1) was added to the medium. Accordingly, laccase activity increased to 1,223 nkat ml(-1). These findings agree with the fact that ethanol induces laccase gene expression in some fungi. Remarkably, lac1 mRNA accumulation and laccase activity also strongly increased in the presence of 25 g of ethanol liter(-1) when lac1 was expressed behind the SC3 or GPD promoter. In the latter case, a maximal laccase activity of 1,393 nkat ml(-1) (i.e., 360 mg liter(-1)) was obtained. Laccase production was further increased in transformants expressing lac1 behind its own promoter or that of GPD by growth in the presence of 40 g of ethanol liter(-1). In this case, maximal activities were 3,900 and 4,660 nkat ml(-1), respectively, corresponding to 1 and 1.2 g of laccase per liter and thus representing the highest laccase activities reported for recombinant fungal strains. These results suggest that P. cinnabarinus may be a host of choice for the production of other proteins as well.
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