ArticleJournal of assisted reproduction and genetics2004
Comparison of maturation, fertilization, development, and gene expression of mouse oocytes grown in vitro and in vivo.
Article in Journal of assisted reproduction and genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- In vitro production of viable eggs from undeveloped oocytes in mouse preantral follicles by reconstructing granulosa cell-oocyte complexes†.Biology of reproduction · 2024Article
- Pronuclear transfer rescues poor embryo development ofHuman reproduction open · 2024Article
- Review
- Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates.Animals : an open access journal from MDPI · 2023Article
- An interpretable and versatile machine learning approach for oocyte phenotyping.Journal of cell science · 2022Article
- Clathrin Heavy Chain 1 Plays Essential Roles During Oocyte Meiotic Spindle Formation and Early Embryonic Development in Sheep.Frontiers in cell and developmental biology · 2021Article
- Identification of a New QTL Region on Mouse Chromosome 1 Responsible for Male Hypofertility: Phenotype Characterization and Candidate Genes.International journal of molecular sciences · 2020Article
- Global, Survival, and Apoptotic Transcriptome during Mouse and Human Early Embryonic Development.BioMed research international · 2018Article
- Defective sperm head decondensation undermines the success of ICSI in the bovine.Reproduction (Cambridge, England) · 2017Article
- Microfluidic analysis of oocyte and embryo biomechanical properties to improve outcomes in assisted reproductive technologies.Molecular human reproduction · 2017Review
- Effect of acteoside on the re-localization and abnormal morphology of mitochondria in porcine oocytes during in vitro maturation.Journal of assisted reproduction and genetics · 2016Article
- Epidermal growth factor-mediated mitogen-activated protein kinase3/1 pathway is conducive to in vitro maturation of sheep oocytes.PloS one · 2015Article
- Expression of G protein estrogen receptor (GPER) on membrane of mouse oocytes during maturation.Journal of assisted reproduction and genetics · 2013Article
- Raman micro-spectroscopy can be used to investigate the developmental stage of the mouse oocyte.PloS one · 2013Article
- In vitro follicle growth under non-attachment conditions and decreased FSH levels reduces Lhcgr expression in cumulus cells and promotes oocyte developmental competence.Journal of assisted reproduction and genetics · 2012Article
- Reduced developmental competence of immature, in-vitro matured and postovulatory aged mouse oocytes following IVF and ICSI.Reproductive biology and endocrinology : RB&E · 2008Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the difference of in vitro and in vivo grown oocytes, we compared maturation, fertilization, development, and maternal gene expression from both in vitro and in vivo grown mouse oocytes.
methodsThe preantral follicles isolated from 12-day-old mice were cultured on Transwell-COL membrane inserts. After culture, maturation, fertilization, and developmental rates were assessed. RT-PCR (reverse transcription-polymerase chain reaction) was performed to examine the expression of beta-actin, GDF-9, and IGF-II in matured oocytes.
resultsNo difference in the nuclear maturation was detected between in vitro and in vivo grown oocytes, but the mean oocyte diameter of the in vitro group was smaller than that of the in vivo group. The fertilization rate was significantly lower in the in vitro group than in the in vivo group (p < 0.05). The capacities of in vitro grown oocyte to cleave and develop to blastocysts were significantly lower than those of the in vivo grown oocytes (p < 0.001). Moreover, blastocyst of in vitro group had fewer total cells than those of in vivo group (p < 0.05). In regards to the expression of genes in mature oocytes, growth differentiation factor-9 (GDF-9) expression was similar between the two groups, but beta-actin was significantly reduced in the in vitro group compared to the in vivo group. Particularly, the expression of insulin-like growth factor II (IGF-II) was not found in the in vitro grown oocytes.
conclusionsThese results showed that in vitro grown oocytes did not have the same developmental capacity as in vivo grown oocytes. We assume that the aberrant expression of maternal-derived genes in the in vitro grown oocytes may cause the poor embryo viability.
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