ArticleWorld journal of gastroenterology2004
Real-time determination of human telomerase reverse transcriptase mRNA in gastric cancer.
Article in World journal of gastroenterology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Differential Expression of Non-Shelterin Genes Associated with High Telomerase Levels and Telomere Shortening in Plasma Cell Disorders.PloS one · 2015Trial
- Article
- Genomic and epigenomic EBF1 alterations modulate TERT expression in gastric cancer.The Journal of clinical investigation · 2020Article
- Expression pattern of hTERT telomerase subunit gene in different stages of chronic myeloid leukemia.Molecular biology reports · 2014Article
- Telomerase and hTERT: can they serve as markers for gastric cancer diagnosis?World journal of gastroenterology · 2014Article
- Prognostic role of telomerase activity in gastric adenocarcinoma: A meta-analysis.Experimental and therapeutic medicine · 2012Article
- Modulation of human serotonin transporter expression by 5-HTTLPR in colon cells.International journal of molecular sciences · 2011Article
- Article
- Chikungunya disease in nonhuman primates involves long-term viral persistence in macrophages.The Journal of clinical investigation · 2010Article
- Dynamics of viral replication in blood and lymphoid tissues during SIVmac251 infection of macaques.Retrovirology · 2009Article
- Infection of semen-producing organs by SIV during the acute and chronic stages of the disease.PloS one · 2008Article
- Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma.Molecular medicine (Cambridge, Mass.)Article
- Telomerase Expression Related with Poor Immune Response to HCV Core Antigen in Egyptian HCV Patients' PBMCs.Journal of clinical and experimental hepatologyArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo set up a real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay, to detect human telomerase reverse transcriptase (hTERT) messenger RNA in gastric carcinomas, and to evaluate quantitative determination of hTERT mRNA in the diagnostic value of gastric carcinomas, and to analyze the correlation between the expression level of hTERT mRNA and clinicopathological parameters in patients with gastric cancer.
methodsA real-time quantitative RT-PCR (RQ-PCR) based on TaqMan fluorescence methodology and the LightCycler system was used to quantify the full range of hTERT mRNA copy numbers in 35 samples of gastric carcinomas and corresponding adjacent non-cancerous tissues. The normalized hTERT (NhTERT) was standardized by quantifying the number of GAPDH transcripts as internal control and expressed as 100X (hTERT/GAPDH) ratio. Variables were analyzed by the Student's t-test, chi2 test and Fisher's exact test.
resultsNhTERT from gastric carcinomas and corresponding adjacent non-cancerous tissues was 6.27+/-0.89 and 0.93+/-0.18, respectively (t = 12.76, P<0.001). There was no significant association between gastric cancer hTERT mRNA expression level and patient's age, gender, tumor size, location and stage (pTNM), but a significant correlation was found between hTERT mRNA expression level in gastric carcinomas and the degree of differentiation.
conclusionQuantitative determination of hTERT mRNA by RQ-PCR is a rapid and sensitive method. hTERT might be a potential biomarker for the early detection of gastric cancer.
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