ReviewCellular and molecular life sciences : CMLS2004
Immortalization protocols used in cell culture models of human breast morphogenesis.
Review in Cellular and molecular life sciences : CMLS, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Shaping the Future Plate: Exploring the Safety Concerns of Cultivated and Conventional Meat Production.Physiological research · 2026Review
- Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer.Frontiers in cell and developmental biology · 2023Article
- SV40T/E6E7-Induced Proliferation Is Involved in the Activity of E2F3 in Bovine Mammary Epithelial Cells.Animals : an open access journal from MDPI · 2022Article
- Establishment and characterization of an immortalized epithelial cell line from human gallbladder.Frontiers in oncology · 2022Article
- Establishment of a sheep immortalization cell line for generating and amplifying Orf virus recombinants.Frontiers in veterinary science · 2022Article
- Detection of phenotype-specific therapeutic vulnerabilities in breast cells using a CRISPR loss-of-function screen.Molecular oncology · 2021Article
- Establishment and characterization of a new spontaneously immortalized ERScientific reports · 2021Article
- Establishment of an immortalized stromal cell line derived from human Endometriotic lesion.Reproductive biology and endocrinology : RB&E · 2020Article
- Development of a NovelFrontiers in cellular and infection microbiology · 2019Article
- A novel method for banking stem cells from human exfoliated deciduous teeth: lentiviral TERT immortalization and phenotypical analysis.Stem cell research & therapy · 2016Article
- Comparison of methods for the isolation of human breast epithelial and myoepithelial cells.Frontiers in cell and developmental biology · 2015Article
- Two methods for establishing primary human endometrial stromal cells from hysterectomy specimens.Journal of visualized experiments : JoVE · 2014Article
- Immortalization of human adipose-derived stromal cells: production of cell lines with high growth rate, mesenchymal marker expression and capability to secrete high levels of angiogenic factors.Stem cell research & therapy · 2014Article
- Modeling platinum sensitive and resistant high-grade serous ovarian cancer: development and applications of experimental systems.Frontiers in oncology · 2014Review
- Association of Notch1 with vasculogenic mimicry in human hepatocellular carcinoma cell lines.International journal of clinical and experimental pathology · 2014Article
- Drug delivery characteristics of the progenitor bronchial epithelial cell line VA10.Pharmaceutical research · 2013Article
- Three-dimensional culture of human breast epithelial cells: the how and the why.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Methods of cell purification: a critical juncture for laboratory research and translational science.Cells, tissues, organs · 2012Article
- Mammary transplantation of stromal cells and carcinoma cells in C57BL/6J mice.Journal of visualized experiments : JoVE · 2011Article
- Selection for EGFR gene amplification in a breast epithelial cell line with basal-like phenotype and hereditary background.In vitro cellular & developmental biology. Animal · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Defining the key players in normal breast differentiation is instrumental to understanding how morphogenesis becomes defective during breast cancer progression. During the past 2 decades much effort has been devoted to the development of technologies for purification and expansion of primary human breast cells in culture and optimizing a relevant microenvironment, which may help to define the niche that regulates breast differentiation and morphogenesis. In contrast to the general property of cancer, normal human cells have a finite lifespan. After a defined number of population doublings, normal cells enter an irreversible proliferation-arrested state referred to as replicative senescence. To overcome this obstacle for continuous long-term studies, replicative senescence can be bypassed by treatment of cells with chemical agents such as benzopyrene, by radiation or by transfection with viral oncogenes or the gene for human telomerase (human telomerase reverse transcriptase, hTERT). A drawback of some of these protocols is a concurrent introduction of chromosomal changes, which sometimes leads to a transformed phenotype and selection of a subpopulation, which may not be representative of the tissue of origin. In recent years, we have sought to establish immortalized primary breast cells, which retain crucial characteristics of their original in situ tissue pattern. This review discusses various approaches to immortalization of breast-derived epithelial and stromal cells and the application of such cell lines for studies on human breast morphogenesis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.