ArticleThe EMBO journal2004
Functional interaction between p90Rsk2 and Emi1 contributes to the metaphase arrest of mouse oocytes.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- Supplementation with Eupatilin during In Vitro Maturation Improves Porcine Oocyte Developmental Competence by Regulating Oxidative Stress and Endoplasmic Reticulum Stress.Animals : an open access journal from MDPI · 2024Article
- Identification and Characterization ofBiomolecules · 2023Article
- Quercetin protects porcine oocytes fromFrontiers in cell and developmental biology · 2022Article
- Importance of ERK1/2 in Regulation of Protein Translation during Oocyte Meiosis.International journal of molecular sciences · 2018Review
- Article
- Roles of MAPK and spindle assembly checkpoint in spontaneous activation and MIII arrest of rat oocytes.PloS one · 2012Article
- Article
- Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC(Cdh1).The Journal of cell biology · 2007Article
- The human cumulus--oocyte complex gene-expression profile.Human reproduction (Oxford, England) · 2006Article
- Mammalian Emi2 mediates cytostatic arrest and transduces the signal for meiotic exit via Cdc20.The EMBO journal · 2006Article
- Regulation of p90RSK phosphorylation by SARS-CoV infection in Vero E6 cells.FEBS letters · 2006Article
- CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- p90Rsk is not involved in cytostatic factor arrest in mouse oocytes.The Journal of cell biology · 2005Article
- A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggs.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vertebrate eggs arrest at metaphase of the second meiotic division before fertilization under the effect of a cytostatic factor (CSF). This arrest is established during oocyte maturation by the MAPK kinase module, comprised of Mos, MEK, MAPKs and p90Rsk. Maintenance of CSF arrest at metaphase requires inhibitors of the anaphase-promoting complex (APC) like Emi1, which sequesters the APC activator Cdc20. Although it was proposed that the Mos pathway and Emi1 act independently, neither one alone is sufficient to entirely reproduce CSF arrest. Herein we demonstrate that p90Rsk2 associates with and phosphorylates Emi1 upstream of the binding region for Cdc20, thus stabilizing their interaction. Experiments in transfected cells and two-cell embryos indicate that Emi1 and p90Rsk2 cooperate to induce the metaphase arrest. Moreover, oocyte maturation was impaired by interfering with the interaction between p90Rsk2 and Emi1 or by RNA interference of Emi1. Our results indicate that p90Rsk2 and Emi1 functionally interact during oocyte maturation and that the Mos pathway establishes CSF activity through stabilization of an APC-inhibitory complex composed by Emi1 and Cdc20 before fertilization.
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