ArticleRNA (New York, N.Y.)2004
Generation of a catalytic module on a self-folding RNA.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Selection for Molecularly Complementary Modules (MCMs) Drives the Origins and Evolution of Pleiofunctional, Epistatic Interactomes (PEIs).Life (Basel, Switzerland) · 2026Review
- A combinatorial method to isolate short ribozymes from complex ribozyme libraries.Nucleic acids research · 2020Article
- RNA modularity for synthetic biology.F1000prime reports · 2013Review
- Computational approaches toward the design of pools for the in vitro selection of complex aptamers.RNA (New York, N.Y.) · 2010Article
- Generation and development of RNA ligase ribozymes with modular architecture through "design and selection".Molecules (Basel, Switzerland) · 2010Review
- Computational generation and screening of RNA motifs in large nucleotide sequence pools.Nucleic acids research · 2010Article
- Evolutionary optimization of a modular ligase ribozyme: a small catalytic unit and a hairpin motif masking an element that could form an inactive structure.Nucleic acids research · 2010Article
- Group I introns and inteins: disparate origins but convergent parasitic strategies.Journal of bacteriology · 2009Review
- A designed RNA selection: establishment of a stable complex between a target and selectant RNA via two coordinated interactions.Nucleic acids research · 2009Article
- A natural ribozyme with 3',5' RNA ligase activity.Nature chemical biology · 2009Article
- Comprehensive features of natural and in vitro selected GNRA tetraloop-binding receptors.Nucleic acids research · 2008Article
- Emergence of a fast-reacting ribozyme that is capable of undergoing continuous evolution.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A computational proposal for designing structured RNA pools for in vitro selection of RNAs.RNA (New York, N.Y.) · 2007Article
- In vitro RNA random pools are not structurally diverse: a computational analysis.RNA (New York, N.Y.) · 2005Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is theoretically possible to obtain a catalytic site of an artificial ribozyme from a random sequence consisting of a limited numbers of nucleotides. However, this strategy has been inadequately explored. Here, we report an in vitro selection technique that exploits modular construction of a structurally constrained RNA to acquire a catalytic site for RNA ligation from a short random sequence. To practice the selection, a sequence of 30 nucleotides was located close to the putative reaction site in a derivative of a naturally occurring self-folding RNA whose crystal structure is known. RNAs whose activity depended on the starting three-dimensional structure were selected with 3'-5' ligation specificity, indicating that the strategy can be used to acquire a variety of catalytic sites and other functional RNA modules.
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Registered trials
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