ArticleMolecular biology of the cell2005
Requirement of the Caenorhabditis elegans RapGEF pxf-1 and rap-1 for epithelial integrity.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- A PDZ-RapGEF promotes synaptic development in Caenorhabditis elegans through a Rap/Rac signaling pathway.Development (Cambridge, England) · 2025Article
- Syndapin and GTPase RAP-1 control endocytic recycling via RHO-1 and non-muscle myosin II.Current biology : CB · 2023Article
- PXF-1 promotes synapse development at the neuromuscular junction inFrontiers in molecular neuroscience · 2022Article
- A simple strategy for addition of degron tags to endogenous genes harboring prior insertions of fluorescent protein.microPublication biology · 2022Article
- Article
- Article
- Alteration in cellular acetylcholine influences dauer formation in Caenorhabditis elegans.BMB reports · 2014Article
- A novel sphingolipid-TORC1 pathway critically promotes postembryonic development in Caenorhabditis elegans.eLife · 2013Article
- Ras effector switching promotes divergent cell fates in C. elegans vulval patterning.Developmental cell · 2011Article
- Control of cell adhesion dynamics by Rap1 signaling.Current opinion in cell biology · 2009Review
- Article
- Beta-catenin asymmetry is regulated by PLA1 and retrograde traffic in C. elegans stem cell divisions.The EMBO journal · 2008Article
- RAP-1 and the RAL-1/exocyst pathway coordinate hypodermal cell organization in Caenorhabditis elegans.The EMBO journal · 2007Article
- The Rap GTPase activator Drosophila PDZ-GEF regulates cell shape in epithelial migration and morphogenesis.Molecular and cellular biology · 2007Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Rap-pathway has been implicated in various cellular processes but its exact physiological function remains poorly defined. Here we show that the Caenorhabditis elegans homologue of the mammalian guanine nucleotide exchange factors PDZ-GEFs, PXF-1, specifically activates Rap1 and Rap2. Green fluorescent protein (GFP) reporter constructs demonstrate that sites of pxf-1 expression include the hypodermis and gut. Particularly striking is the oscillating expression of pxf-1 in the pharynx during the four larval molts. Deletion of the catalytic domain from pxf-1 leads to hypodermal defects, resulting in lethality. The cuticle secreted by pxf-1 mutants is disorganized and can often not be shed during molting. At later stages, hypodermal degeneration is seen and animals that reach adulthood frequently die with a burst vulva phenotype. Importantly, disruption of rap-1 leads to a similar, but less severe phenotype, which is enhanced by the simultaneous removal of rap-2. In addition, the lethal phenotype of pxf-1 can be rescued by expression of an activated version of rap-1. Together these results demonstrate that the pxf-1/rap pathway in C. elegans is required for maintenance of epithelial integrity, in which it probably functions in polarized secretion.
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