ArticleBiomaterials2005
DNA delivery from hyaluronic acid-collagen hydrogels via a substrate-mediated approach.
Article in Biomaterials, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 164 citations in OpenAlex.
- Biomaterial-assisted gene therapy for bone repair.Materials today. Bio · 2026Review
- Carbohydrate-Based Hydrogels: Weaving Nature's Versatility into Biomedical Innovation.International journal of nanomedicine · 2026Review
- Multifunctional DNA-Collagen Biomaterials: Developmental Advances and Biomedical Applications.ACS biomaterials science & engineering · 2025Review
- Polysaccharide-Based Hydrogels and Their Application as Drug Delivery Systems in Cancer Treatment: A Review.Journal of functional biomaterials · 2023Review
- Collagen- and hyaluronic acid-based hydrogels and their biomedical applications.Materials science & engineering. R, Reports : a review journal · 2021Article
- Hyaluronan as a Prominent Biomolecule with Numerous Applications in Medicine.International journal of molecular sciences · 2021Review
- Polysaccharide-Based In Situ Self-Healing Hydrogels for Tissue Engineering Applications.Polymers · 2020Review
- Hyaluronic Acid as a Component of Natural Polymer Blends for Biomedical Applications: A Review.Molecules (Basel, Switzerland) · 2020Review
- Screening a chemically defined extracellular matrix mimetic substrate library to identify substrates that enhance substrate-mediated transfection.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Three-dimensional aligned nanofibers-hydrogel scaffold for controlled non-viral drug/gene delivery to direct axon regeneration in spinal cord injury treatment.Scientific reports · 2017Article
- Engineered Hydrogels for Local and Sustained Delivery of RNA-Interference Therapies.Advanced healthcare materials · 2017Review
- Integration of growth factor gene delivery with collagen-triggered wound repair cascades using collagen-mimetic peptides.Bioengineering & translational medicine · 2016Article
- Growth factor-eluting technologies for bone tissue engineering.Drug delivery and translational research · 2016Review
- MicroRNA delivery for regenerative medicine.Advanced drug delivery reviews · 2015Review
- Effective release of a broad spectrum antibiotic from elastin-like polypeptide-collagen composite.Journal of biomedical materials research. Part A · 2015Article
- Gene delivery strategies to promote spinal cord repair.Biomarker insights · 2015Review
- Osteogenic potential of BMP-2-releasing self-assembled membranes.Tissue engineering. Part A · 2013Article
- Matrix-based gene delivery for tissue repair.Current opinion in biotechnology · 2013Review
- Gene therapy vectors with enhanced transfection based on hydrogels modified with affinity peptides.Biomaterials · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Efficient and controlled gene delivery from biodegradable materials can be employed to stimulate cellular processes that lead to tissue regeneration. In this report, a substrate-mediated approach was developed to deliver DNA from hyaluronic acid-collagen hydrogels. The hydrogels were formed by crosslinking HA with poly(ethylene glycol) diglycidyl ether. Poly(ethylene imine)(PEI)/DNA complexes were immobilized to the substrate using either biotin/neutravidin or non-specific adsorption. Complexes were formed in the presence or absence of salt to regulate complex size, and resulted in complexes with z-average diameters of 1221.7 +/- 152.3 and 139.4 +/- 1.3 nm, respectively. During 48-h incubation in PBS or hyaluronidase, DNA was released slowly from the hydrogel substrate (<30% of immobilized DNA), which was enhanced by incubation with conditioned media (approximately 50% of immobilized DNA). Transgene expression mediated by immobilized, large diameter complexes was 3 to 7-fold greater than for small diameter complexes. However, the percentage of cells expressing the transgene was greater for small diameter complexes (48.7%) than for large diameter complexes (22.3%). Spatially controlled gene transfer was achieved by topographically patterning the hydrogel to pattern cell adhesion. Biomaterial-based gene delivery can be applicable to numerous tissue engineering applications, or as a tool to examine tissue formation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.