ArticleNucleic acids research2004
Overexpression of human DNA polymerase mu (Pol mu) in a Burkitt's lymphoma cell line affects the somatic hypermutation rate.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Molecular mechanisms of DNA lesion and repair during antibody somatic hypermutation.Science China. Life sciences · 2024Review
- Article
- Mechanism of genome instability mediated by human DNA polymerase mu misincorporation.Nature communications · 2021Article
- Polμ tumor variants decrease the efficiency and accuracy of NHEJ.Nucleic acids research · 2017Article
- Analysis of the relationship between coexpression domains and chromatin 3D organization.PLoS computational biology · 2017Article
- Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina.Scientific reports · 2016Article
- Article
- Molecular basis for DNA double-strand break annealing and primer extension by an NHEJ DNA polymerase.Cell reports · 2013Article
- Biological and therapeutic relevance of nonreplicative DNA polymerases to cancer.Antioxidants & redox signaling · 2013Review
- Ribonucleotides and manganese ions improve non-homologous end joining by human Polμ.Nucleic acids research · 2013Article
- DNA expansions generated by human Polμ on iterative sequences.Nucleic acids research · 2013Article
- Yeast pol4 promotes tel1-regulated chromosomal translocations.PLoS genetics · 2013Article
- Modulation of Pleurodeles waltl DNA polymerase mu expression by extreme conditions encountered during spaceflight.PloS one · 2013Article
- Error-prone DNA repair activity during somatic hypermutation in shark B lymphocytes.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Limited terminal transferase in human DNA polymerase mu defines the required balance between accuracy and efficiency in NHEJ.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A role for DNA polymerase mu in the emerging DJH rearrangements of the postgastrulation mouse embryo.Molecular and cellular biology · 2009Article
- Article
- Article
- Characterization of terminal deoxynucleotidyl transferase and polymerase mu in zebrafish.Immunogenetics · 2007Article
- A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA polymerase mu (Pol mu) is a DNA-dependent DNA polymerase closely related to terminal deoxynucleotidyl transferase (TdT), and prone to induce template/primer misalignments and misincorporation. In addition to a proposed general role in non-homologous end joining of double-strand breaks, its mutagenic potential and preferential expression in secondary lymphoid tissues support a role in somatic hypermutation (SHM) of immunoglobulin genes. Here, we show that human Pol mu protein is expressed in the nucleus of centroblasts obtained from human tonsils, forming a characteristic foci pattern resembling that of other DNA repair proteins in response to DNA damage. Overexpression of human Pol mu in Ramos cells, in which the SHM process is constitutive, augmented the somatic mutations specifically at the variable (V) region of the immunoglobulin genes. The nature of the mutations introduced, mostly base substitutions, supports the contribution of Pol mu to mutation of G and C residues during SHM. In vitro analysis of Pol mu misincorporation on specific templates, that mimic DNA repair intermediates and correspond to mutational hotspots, indicated that many of the mutations observed in vivo can be explained by the capacity of Pol mu to induce transient template/primer misalignments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.