ArticleNucleic acids research2004
Site-selective and hydrolytic two-strand scission of double-stranded DNA using Ce(IV)/EDTA and pseudo-complementary PNA.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Emerging tools in plant genome editing.Frontiers in genome editing · 2025Review
- Ce-based solid-phase catalysts for phosphate hydrolysis as new tools for next-generation nanoarchitectonics.Science and technology of advanced materials · 2023Article
- Fabrication of highly active phosphatase-like fluorescent cerium-doped carbon dots forRSC advances · 2020Article
- Cut-and-Paste of DNA Using an Artificial Restriction DNA Cutter.International journal of molecular sciences · 2013Article
- Enhanced annealing of mismatched oligonucleotides using a novel melting curve assay allows efficient in vitro discrimination and restriction of a single nucleotide polymorphism.BMC biotechnology · 2011Article
- New macrocyclic terbium(III) complex for use in RNA footprinting experiments.Journal of the American Chemical Society · 2009Article
- Chiral introduction of positive charges to PNA for double-duplex invasion to versatile sequences.Nucleic acids research · 2008Article
- Crystal structure of Ce(IV)/dipicolinate complex as catalyst for DNA hydrolysis.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2008Article
- Site-directed gene mutation at mixed sequence targets by psoralen-conjugated pseudo-complementary peptide nucleic acids.Nucleic acids research · 2007Article
- Chemical modification of Ce(IV)/EDTA-based artificial restriction DNA cutter for versatile manipulation of double-stranded DNA.Nucleic acids research · 2007Article
- Recombination of the GFP gene to the BFP gene using a man-made site-selective DNA cutter.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By combining Ce(IV)/EDTA with two pseudo-complementary peptide nucleic acids (pcPNAs), both strands in double-stranded DNA were site-selectively hydrolyzed at the target site. Either plasmid DNA (4361 bp) or its linearized form was used as the substrate. When two pcPNAs invaded into the double-stranded DNA, only the designated portion in each of the two strands was free from Watson-Crick base pairing with the counterpart DNA or the pcPNA. Upon the treatment of this invasion complex with Ce(IV)/EDTA at 37 degrees C and pH 7.0, both of these single-stranded portions were selectively hydrolyzed at the designated site, resulting in the site-selective two-strand scission of the double-stranded DNA. Furthermore, the hydrolytic scission products were successfully connected with foreign double-stranded DNA by using ligase. The potential of these artificial systems for manipulation of huge DNA has been indicated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.