ArticleProceedings of the National Academy of Sciences of the United States of America2004
Different functions of HetR, a master regulator of heterocyst differentiation in Anabaena sp. PCC 7120, can be separated by mutation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 70 citations in OpenAlex.
- Tradeoffs between phage resistance and nitrogen fixation drive the evolution of genes essential for cyanobacterial heterocyst functionality.The ISME journal · 2024Article
- Terminal heterocyst differentiation in the Anabaena patA mutant as a result of post-transcriptional modifications and molecular leakage.PLoS computational biology · 2022Article
- Mathematical models of nitrogen-fixing cell patterns in filamentous cyanobacteria.Frontiers in cell and developmental biology · 2022Review
- Cell Cycle, Filament Growth and Synchronized Cell Division in Multicellular Cable Bacteria.Frontiers in microbiology · 2021Article
- HetL, HetR and PatS form a reaction-diffusion system to control pattern formation in the cyanobacteriumeLife · 2020Article
- Carbon Transfer from the Host Diatom Enables Fast Growth and High Rate of NPlants (Basel, Switzerland) · 2020Article
- Origins of peptidases.Biochimie · 2019Article
- Article
- Cyanobacterial Septal Junctions: Properties and Regulation.Life (Basel, Switzerland) · 2018Review
- Manipulation of Pattern of Cell Differentiation in aLife (Basel, Switzerland) · 2018Article
- Formation and maintenance of nitrogen-fixing cell patterns in filamentous cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Structural insights into HetR-PatS interaction involved in cyanobacterial pattern formation.Scientific reports · 2015Article
- Spatial fluctuations in expression of the heterocyst differentiation regulatory gene hetR in Anabaena filaments.PLoS genetics · 2015Article
- An integrative approach for modeling and simulation of heterocyst pattern formation in cyanobacteria filaments.PLoS computational biology · 2015Article
- Deep sequencing of HetR-bound DNA reveals novel HetR targets in Anabaena sp. strain PCC7120.BMC microbiology · 2014Article
- Article
- ppGpp metabolism is involved in heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120.Journal of bacteriology · 2013Article
- Restricted cellular differentiation in cyanobacterial filaments.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Identification of the HetR recognition sequence upstream of hetZ in Anabaena sp. strain PCC 7120.Journal of bacteriology · 2012Article
- Mutations in genes patA and patL of Anabaena sp. strain PCC 7120 result in similar phenotypes, and the proteins encoded by those genes may interact.Journal of bacteriology · 2011Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
The HetR protein has long been recognized as a key player in the regulation of heterocyst development. HetR is known to possess autoproteolytic and DNA-binding activities. During a search for mutants of Anabaena sp. PCC 7120 that can overcome heterocyst suppression caused by overexpression of the patS gene, which encodes a negative regulator of differentiation, a bypass mutant strain, S2-45, was isolated that produced a defective pattern (Pat phenotype) of irregularly spaced single and multiple contiguous heterocysts (Mch phenotype) in combined nitrogen-free medium. Analysis of the S2-45 mutant revealed a R223W mutation in HetR, and reconstruction in the wild-type background showed that this mutation was responsible for the Mch phenotype and resistance not only to overexpressed patS, but also to overexpressed hetN, another negative regulator of differentiation. Ectopic overexpression of the hetRR223W allele in the hetRR223W background resulted in a conditionally lethal (complete differentiation) phenotype. Analysis of the heterocyst pattern in the hetRR223W mutant revealed that heterocysts differentiate essentially randomly along filaments, indicating that this mutation results in an active protein that is insensitive to the major signals governing heterocyst pattern formation. These data provide genetic evidence that, apart from being an essential activator of differentiation, HetR plays a central role in the signaling pathway that controls the heterocyst pattern.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.