ArticleGenome research2004
DNA amplification method tolerant to sample degradation.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- Evaluating the Benefits and Limits of Multiple Displacement Amplification With Whole-Genome Oxford Nanopore Sequencing.Molecular ecology resources · 2025Article
- Development and Clinical Validation of Molecular Subgrouping in Medulloblastoma by Targeted Methylation Sequencing.Asian Pacific journal of cancer prevention : APJCP · 2025Article
- "Evaluating the Benefits and Limits of Multiple Displacement Amplification with Whole-Genome Oxford Nanopore Sequencing".bioRxiv : the preprint server for biology · 2024Article
- New Perspectives for Whole Genome Amplification in Forensic STR Analysis.International journal of molecular sciences · 2022Review
- A low cost and input tailing method of quality control on multiple annealing, and looping-based amplification cycles-based whole-genome amplification products.Journal of clinical laboratory analysis · 2019Article
- Understanding preanalytical variables and their effects on clinical biomarkers of oncology and immunotherapy.Seminars in cancer biology · 2018Review
- Systematic assessment of the performance of whole-genome amplification for SNP/CNV detection and β-thalassemia genotyping.Journal of human genetics · 2018Article
- Comparative study of whole genome amplification and next generation sequencing performance of single cancer cells.Oncotarget · 2017Article
- Template-dependent multiple displacement amplification for profiling human circulating RNA.BioTechniques · 2017Article
- MitoRCA-seq reveals unbalanced cytocine to thymine transition in Polg mutant mice.Scientific reports · 2015Article
- Article
- Whole genome amplification of degraded and nondegraded DNA for forensic purposes.International journal of legal medicine · 2013Article
- Comparison of whole genome amplification methods for analysis of DNA extracted from microdissected early breast lesions in formalin-fixed paraffin-embedded tissue.ISRN oncology · 2012Article
- Microarray-based evaluation of whole-community genome DNA amplification methods.Applied and environmental microbiology · 2011Article
- Multiplex amplification coupled with COLD-PCR and high resolution melting enables identification of low-abundance mutations in cancer samples with low DNA content.The Journal of molecular diagnostics : JMD · 2011Article
- Frequent alteration of MLL3 frameshift mutations in microsatellite deficient colorectal cancer.PloS one · 2011Article
- Multiple displacement amplification for complex mixtures of DNA fragments.BMC genomics · 2008Article
- Performance of whole-genome amplified DNA isolated from serum and plasma on high-density single nucleotide polymorphism arrays.The Journal of molecular diagnostics : JMD · 2008Article
- DNA degradation test predicts success in whole-genome amplification from diverse clinical samples.The Journal of molecular diagnostics : JMD · 2007Article
- Large fragment Bst DNA polymerase for whole genome amplification of DNA from formalin-fixed paraffin-embedded tissues.BMC genomics · 2006Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Despite recent advances in linear whole genome amplification of intact DNA/RNA, amplification of degraded nucleic acids in an unbiased fashion remains a serious challenge for genetic diagnosis. We describe a new whole genome amplification procedure, RCA-RCA (Restriction and Circularization-Aided Rolling Circle Amplification), which retains the allelic differences among degraded amplified genomes while achieving almost complete genome coverage. RCA-RCA utilizes restriction digestion and whole genome circularization to generate genomic sequences amenable to rolling circle amplification. When intact genomic DNA is used, RCA-RCA retains gene-amplification differences (twofold or higher) between complex genomes on a genome-wide scale providing highly improved concordance with unamplified material as compared with other amplification methodologies including multiple displacement amplification. Using RCA-RCA, formalin-fixed samples of modest or substantial DNA degradation were successfully amplified and screened via array-CGH or Taqman PCR that displayed retention of the principal gene amplification features of the original material. Microsatellite analysis revealed that RCA-RCA amplified genomic DNA is representative of the original material at the nucleotide level. Amplification of cDNA is successfully performed via RCA-RCA and results to unbiased gene expression analysis (R(2) = 0.99). The simplicity and universal applicability of RCA-RCA make it a powerful new tool for genome analysis with unique advantages over previous amplification technologies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.