ArticleGenome research2004
The molecular structure of the DNA fragments eliminated during chromatin diminution in Cyclops kolensis.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Functions and mechanisms of eukaryotic RNA-guided programmed DNA elimination.Biochemical Society transactions · 2025Review
- Chromatin diminution as a tool to study some biological problems.Comparative cytogenetics · 2024Article
- Article
- Novel selectively amplified DNA sequences in the germline genome of the Japanese hagfish, Eptatretus burgeri.Scientific reports · 2022Article
- Programmed DNA elimination: silencing genes and repetitive sequences in somatic cells.Biochemical Society transactions · 2021Review
- Comparative genome analysis of programmed DNA elimination in nematodes.Genome research · 2017Article
- DNA Remodeling by Strict Partial Endoreplication in Orchids, an Original Process in the Plant Kingdom.Genome biology and evolution · 2017Article
- Characterization of Somatically-Eliminated Genes During Development of the Sea Lamprey (Petromyzon marinus).Molecular biology and evolution · 2016Article
- Programmed DNA elimination in multicellular organisms.Current opinion in genetics & development · 2014Review
- Chromatin diminution in Copepoda (Crustacea): pattern, biological role and evolutionary aspects.Comparative cytogenetics · 2014Article
- Unusual augmentation of germline genome size in Cyclops kolensis (Crustacea, Copepoda): further evidence in support of a revised model of chromatin diminution.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Programmed DNA elimination in Tetrahymena: a small RNA-mediated genome surveillance mechanism.Advances in experimental medicine and biology · 2011Review
- Chromatin Diminution Process Regulates rRNA Gene Copy Number in Freshwater Copepods.Acta naturae · 2010Article
- Highly conserved repeated redundant DNA in two Cyclops species.Doklady. Biochemistry and biophysicsArticle
- Characterization of Cyclops kolensis inter-simple sequence repeats in germline and postdiminution somatic cells.Doklady. Biochemistry and biophysicsArticle
- The conservatism of the genome structure of Cyclops kolensis (Copepoda, Crustacea) populations inhabiting Moscow ponds and Lake Baikal.Doklady. Biochemistry and biophysicsArticle
- EMBRYONIC DEVELOPMENT AND A QUANTITATIVE MODEL OF PROGRAMMED DNA ELIMINATION INJournal of crustacean biology : a quarterly of the Crustacean Society for the publication of research on any aspect of the biology of crustaceaArticle
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Presumptive somatic cells of the copepod Cyclops kolensis specifically eliminate a large fraction of their genome by the process of chromatin diminution. The eliminated DNA (eDNA) remains only in the germline cells. Very little is known about the nature of the sequences eliminated from somatic cells. We cloned a fraction of the eDNA and sequenced 90 clones that total 32 kb. The following organizational patterns were demonstrated for the eDNA sequences. All do not contain open reading frames. Each fragment contains 1-3 families of short repeats (10-30 bp) highly homologous within families (87%-100%). Most repeats are separated by spacers up to 50 bp long. Homologous regions were found between fragments, motifs from 15-300 bp in length. Among fragments there occur groups in which the same motifs are ordered in the same fashion. However, spacers between the motifs differ in length and nucleotide composition. Ubiquitous motifs (those occurring in all fragments) were identified. Analysis of motifs revealed submotifs, each occurring within several motifs. Thus, motifs may be regarded as mosaic structures composed of submotifs (short repeats). Taken together, the results provide evidence of a high organizational ordering of the DNA sequences restricted to the germline. With this in mind, it appears incorrect to refer to this part of the genome as junk. Moreover, eDNA is redundant for only the somatic cells-its function is to be sought in germline cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.