ArticleGenetics2005
Analysis of conditional paralytic mutants in Drosophila sarco-endoplasmic reticulum calcium ATPase reveals novel mechanisms for regulating membrane excitability.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 79 citations in OpenAlex.
- Transcriptomic Profiling of Bean AphidInsects · 2024Article
- Wolfram syndrome 1 regulates sleep in dopamine receptor neurons by modulating calcium homeostasis.PLoS genetics · 2023Article
- Drosophila SPG12 ortholog, reticulon-like 1, governs presynaptic ER organization and Ca2+ dynamics.The Journal of cell biology · 2023Article
- Neprilysins regulate muscle contraction and heart function via cleavage of SERCA-inhibitory micropeptides.Nature communications · 2022Article
- Piezo buffers mechanical stress via modulation of intracellular CaFrontiers in physiology · 2022Article
- Vmp1, Vps13D, and Marf/Mfn2 function in a conserved pathway to regulate mitochondria and ER contact in development and disease.Current biology : CB · 2021Article
- Ral function in muscle is required for flight maintenance inSmall GTPases · 2020Article
- Dstac Regulates Excitation-Contraction Coupling inFrontiers in physiology · 2020Article
- Axonal Endoplasmic Reticulum Dynamics and Its Roles in Neurodegeneration.Frontiers in neuroscience · 2020Review
- Identification of a pathogenic mutation in ATP2A1 via in silico analysis of exome data for cryptic aberrant splice sites.Molecular genetics & genomic medicine · 2019Article
- Reduced SERCA Function Preferentially Affects Wnt Signaling by Retaining E-Cadherin in the Endoplasmic Reticulum.Cell reports · 2019Article
- SERCA is critical to control the Bowditch effect in the heart.Scientific reports · 2018Article
- THADA Regulates the Organismal Balance between Energy Storage and Heat Production.Developmental cell · 2017Article
- Small heat shock proteins mediate cell-autonomous and -nonautonomous protection in a Drosophila model for environmental-stress-induced degeneration.Disease models & mechanisms · 2016Article
- Probing the Fractal Pattern of Heartbeats in Drosophila Pupae by Visible Optical Recording System.Scientific reports · 2016Article
- Store-independent modulation of Ca(2+) entry through Orai by Septin 7.Nature communications · 2016Article
- The effects of ER morphology on synaptic structure and function in Drosophila melanogaster.Journal of cell science · 2016Article
- Article
- Ionic leakage underlies a gain-of-function effect of dominant disease mutations affecting diverse P-type ATPases.Nature genetics · 2014Article
- Article
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Authors and funding
8 authors at 6 institutions in 4 countries.
Funding
Abstract
Individual contributions made by different calcium release and sequestration mechanisms to various aspects of excitable cell physiology are incompletely understood. SERCA, a sarco-endoplasmic reticulum calcium ATPase, being the main agent for calcium uptake into the ER, plays a central role in this process. By isolation and extensive characterization of conditional mutations in the Drosophila SERCA gene, we describe novel roles of this key protein in neuromuscular physiology and enable a genetic analysis of SERCA function. At motor nerve terminals, SERCA inhibition retards calcium sequestration and reduces the amplitude of evoked excitatory junctional currents. This suggests a direct contribution of store-derived calcium in determining the quantal content of evoked release. Conditional paralysis of SERCA mutants is also marked by prolonged neural activity-driven muscle contraction, thus reflecting the phylogenetically conserved role of SERCA in terminating contraction. Further analysis of ionic currents from mutants uncovers SERCA-dependent mechanisms regulating voltage-gated calcium channels and calcium-activated potassium channels that together control muscle excitability. Finally, our identification of dominant loss-of-function mutations in SERCA indicates novel intra- and intermolecular interactions for SERCA in vivo, overlooked by current structural models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.