Evidence map›Paper›PMID 15520264›Full record

ArticleGenetics2005

Distribution of Activator (Ac) throughout the maize genome for use in regional mutagenesis.

Judith M Kolkman, Liza J Conrad, Phyllis R Farmer, Kristine Hardeman, Kevin R Ahern, Paul E Lewis, Ruairidh J H Sawers, Sara Lebejko, Paul Chomet, Thomas P Brutnell

Abstract read
In one paragraph

Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed.

  1. Progresses of transposon research inSynthetic and systems biotechnology · 2026
    Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Analysis of tandem gene copies in maize chromosomal regions reconstructed from long sequence reads.Proceedings of the National Academy of Sciences of the United States of America · 2016
    Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Distributed simple sequence repeat markers for efficient mapping from maize public mutagenesis populations.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Judith M KolkmanBoyce Thompson Institute, Cornell University, Ithaca, New York 14853, USA.
Liza J Conrad
Phyllis R Farmer
Kristine Hardeman
Kevin R Ahern
Paul E Lewis
Ruairidh J H Sawers
Sara Lebejko
Paul Chomet
Thomas P Brutnell

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A collection of Activator (Ac)-containing, near-isogenic W22 inbred lines has been generated for use in regional mutagenesis experiments. Each line is homozygous for a single, precisely positioned Ac element and the Ds reporter, r1-sc:m3. Through classical and molecular genetic techniques, 158 transposed Ac elements (tr-Acs) were distributed throughout the maize genome and 41 were precisely placed on the linkage map utilizing multiple recombinant inbred populations. Several PCR techniques were utilized to amplify DNA fragments flanking tr-Ac insertions up to 8 kb in length. Sequencing and database searches of flanking DNA revealed that the majority of insertions are in hypomethylated, low- or single-copy sequences, indicating an insertion site preference for genic sequences in the genome. However, a number of Ac transposition events were to highly repetitive sequences in the genome. We present evidence that suggests Ac expression is regulated by genomic context resulting in subtle variations in Ac-mediated excision patterns. These tr-Ac lines can be utilized to isolate genes with unknown function, to conduct fine-scale genetic mapping experiments, and to generate novel allelic diversity in applied breeding programs.

Indexed as

Genome, PlantMutagenesis, InsertionalChromosome MappingChromosomes, PlantCloning, MolecularDNA, PlantDNA Transposable ElementsGene Expression Regulation, PlantGenes, PlantGenes, ReporterGenetic TechniquesGenetic VariationHomozygoteMolecular Sequence DataPolymerase Chain ReactionRepetitive Sequences, Nucleic AcidDNA, PlantDNA Transposable ElementsRetroelements

Identifiers

PMID15520264
PMCPMC1449104

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.