ArticleThe Journal of cell biology2004
A pRb-independent mechanism preserves the postmitotic state in terminally differentiated skeletal muscle cells.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 69 citations in OpenAlex.
- Restoring the Cell Cycle and Proliferation Competence in Terminally Differentiated Skeletal Muscle Myotubes.Cells · 2021Review
- Changes in chromatin accessibility ensure robust cell cycle exit in terminally differentiated cells.PLoS biology · 2019Article
- A defective dNTP pool hinders DNA replication in cell cycle-reactivated terminally differentiated muscle cells.Cell death and differentiation · 2017Article
- Sex hormones establish a reserve pool of adult muscle stem cells.Nature cell biology · 2016Article
- Peroxisome Proliferator-activated Receptor γ Coactivator-1 α Isoforms Selectively Regulate Multiple Splicing Events on Target Genes.The Journal of biological chemistry · 2016Article
- Proliferation of Multiple Cell Types in the Skeletal Muscle Tissue Elicited by Acute p21 Suppression.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- Chronic Replication Problems Impact Cell Morphology and Adhesion of DNA Ligase I Defective Cells.PloS one · 2015Article
- A differential response to newt regeneration extract by C2C12 and primary mammalian muscle cells.Skeletal muscle · 2015Article
- Satellite cells: regenerative mechanisms and applicability in muscular dystrophy.Stem cells international · 2015Review
- Retinoblastoma protein and MyoD function together to effect the repression of Fra-1 and in turn cyclin D1 during terminal cell cycle arrest associated with myogenesis.The Journal of biological chemistry · 2014Article
- The other side of the coin: the tumor-suppressive aspect of oncogenes and the oncogenic aspect of tumor-suppressive genes, such as those along the CCND-CDK4/6-RB axis.Cell cycle (Georgetown, Tex.) · 2014Review
- Epigenetic regulation of myogenic gene expression by heterochromatin protein 1 alpha.PloS one · 2013Article
- Differentiation-associated microRNAs antagonize the Rb-E2F pathway to restrict proliferation.The Journal of cell biology · 2012Article
- Regulation of stem cell plasticity: mechanisms and relevance to tissue biology and cancer.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Review
- Article
- miR-200c is upregulated by oxidative stress and induces endothelial cell apoptosis and senescence via ZEB1 inhibition.Cell death and differentiation · 2011Article
- Rb and p130 control cell cycle gene silencing to maintain the postmitotic phenotype in cardiac myocytes.The Journal of cell biology · 2011Article
- Dedifferentiation, transdifferentiation and reprogramming: three routes to regeneration.Nature reviews. Molecular cell biology · 2011Review
- Transient inactivation of Rb and ARF yields regenerative cells from postmitotic mammalian muscle.Cell stem cell · 2010Article
- Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
In skeletal muscle differentiation, the retinoblastoma protein (pRb) is absolutely necessary to establish definitive mitotic arrest. It is widely assumed that pRb is equally essential to sustain the postmitotic state, but this contention has never been tested. Here, we show that terminal proliferation arrest is maintained in skeletal muscle cells by a pRb-independent mechanism. Acute Rb excision from conditional knockout myotubes caused reexpression of E2F transcriptional activity, cyclin-E and -A kinase activities, PCNA, DNA ligase I, RPA, and MCM2, but did not induce DNA synthesis, showing that pRb is not indispensable to preserve the postmitotic state of these cells. Muscle-specific gene expression was significantly down-regulated, showing that pRb is constantly required for optimal implementation of the muscle differentiation program. Rb-deleted myotubes were efficiently reactivated by forced expression of cyclin D1 and Cdk4, indicating a functionally significant target other than pRb for these molecules. Finally, Rb removal induced no DNA synthesis even in pocket-protein null cells. Thus, the postmitotic state of myotubes is maintained by at least two mechanisms, one of which is pocket-protein independent.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.