ArticleJournal of the American Society for Mass Spectrometry2004
Transport of 13C-oleate in adipocytes measured using multi imaging mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 70 citations in OpenAlex.
- Progress in monitoring molecular, cellular and metabolic dynamics using mass spectrometry imaging.Bioanalysis · 2025Review
- Metabolomics: A useful tool for ischemic stroke research.Journal of pharmaceutical analysis · 2023Review
- Highlighting Functional Mass Spectrometry Imaging Methods in Bioanalysis.Journal of proteome research · 2022Review
- Visualization of Partial Exocytotic Content Release and Chemical Transport into Nanovesicles in Cells.ACS nano · 2022Article
- Use of stable isotope-tagged thymidine and multi-isotope imaging mass spectrometry (MIMS) for quantification of human cardiomyocyte division.Nature protocols · 2021Article
- Single cell metabolomics using mass spectrometry: Techniques and data analysis.Analytica chimica acta · 2021Review
- Chemical Analysis of Single Cells and Organelles.Analytical chemistry · 2021Review
- The Application of Single-Cell Technologies in Cardiovascular Research.Frontiers in cell and developmental biology · 2021Review
- Subcellular Mass Spectrometry Imaging and Absolute Quantitative Analysis across Organelles.ACS nano · 2020Article
- Automated Interpretation and Extraction of Topographic Information from Time of Flight Secondary Ion Mass Spectrometry Data.Scientific reports · 2017Article
- NanoSIMS imaging: an approach for visualizing and quantifying lipids in cells and tissues.Journal of investigative medicine : the official publication of the American Federation for Clinical Research · 2017Review
- An Overview of Human Immunodeficiency Virus Type 1-Associated Common Neurological Complications: Does Aging Pose a Challenge?Journal of Alzheimer's disease : JAD · 2017Review
- Nonalcoholic Fatty Liver Disease: Lipids and Insulin Resistance.Clinics in liver disease · 2016Review
- Microscopy tools for the investigation of intracellular lipid storage and dynamics.Molecular metabolism · 2016Review
- Single-cell imaging mass spectrometry.Current opinion in chemical biology · 2013Review
- Single-cell lipidomics: characterizing and imaging lipids on the surface of individual Aplysia californica neurons with cluster secondary ion mass spectrometry.Analytical chemistry · 2013Article
- Imaging beyond the proteome.Chemical communications (Cambridge, England) · 2012Review
- Mass spectrometry imaging and profiling of single cells.Journal of proteomics · 2012Review
- Lipid imaging with time-of-flight secondary ion mass spectrometry (ToF-SIMS).Biochimica et biophysica acta · 2011Review
- Fatty acid (FFA) transport in cardiomyocytes revealed by imaging unbound FFA is mediated by an FFA pump modulated by the CD36 protein.The Journal of biological chemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
The mechanism of long chain free fatty acid (FFA) transport across cell membranes is under active investigation. Here we describe the use of multi imaging mass spectrometry (MIMS) to monitor intracellular concentrations of FFA and provide new insight into FFA transport in cultured adipocytes. Cells were incubated with 13C-oleate:BSA and either dried directly or dried after washing with a medium deprived of 13C-oleate:BSA. Cells were analyzed with MIMS using a scanning primary Cs+ ion beam and 12C-, 13C-, 12C14N-, 13C14N-) (or 12C 15N-) were imaged simultaneously. From these quantitative images the values of the 13C/ 12C ratios were determined in the intracellular lipid droplets, in the cytoplasm and outside the 3T3F442A adipocytes. The results indicate that after incubation with 13C-oleate:BSA the droplet 13C/ 12C ratio was 15 +/- 6%. This value is about 14-fold higher than the 13C/ 12C terrestrial ratio (1.12%). After washing the 13C-oleate:BSA, the droplet 13C/ 12C ratios decreased to 1.6 +/- 0.1%, about 40% greater than the natural abundance. Results for washed cells indicate that relatively little FFA was esterified. The unwashed cell results, together with the value of the lipid water partition coefficient, reveal that intracellular unbound FFA (FFAu) concentrations were on average about 4.5-fold greater than the extracellular FFAu concentrations. These results are consistent with the possibility that FFA may be pumped into adipocytes against their electro-chemical potential. This work demonstrates that MIMS can be used to image and quantitate stable isotope labeled fatty acid in intracellular lipid droplets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.