ArticleImmunology1992
Biochemical and immunological properties of rat recombinant interleukin-2 and interleukin-4.
Article in Immunology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 49 citations in OpenAlex.
- Listeria monocytogenes infection affects a subset of Ly49-expressing NK cells in the rat.PloS one · 2010Article
- KLRE/I1 and KLRE/I2: a novel pair of heterodimeric receptors that inversely regulate NK cell cytotoxicity.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- The lectin-like receptor KLRE1 inhibits natural killer cell cytotoxicity.The Journal of experimental medicine · 2003Article
- Selective enhancement of systemic Th1 immunity in immunologically immature rats with an orally administered bacterial extract.Infection and immunity · 2001Article
- Advances in animal cell recombinant protein production: GS-NS0 expression system.Cytotechnology · 2000Article
- Article
- Glucocorticoids enhance concanavalin A-induced mitogenic response through the inhibition of nitric oxide production.Immunology · 1997Article
- An autosomal dominant locus, Nka, mapping to the Ly-49 region of a rat natural killer (NK) gene complex, controls NK cell lysis of allogeneic lymphocytes.The Journal of experimental medicine · 1996Article
- Mercuric chloride, a chemical responsible for T helper cell (Th)2-mediated autoimmunity in brown Norway rats, directly triggers T cells to produce interleukin-4.The Journal of clinical investigation · 1995Article
- Activation of CD4+ T cells in the presence of a nondepleting monoclonal antibody to CD4 induces a Th2-type response in vitro.The Journal of experimental medicine · 1995Article
- Characterization of Interleukin-2 (IL-2) receptor expression and action of IL-2 and IL-6 on normal anterior pituitary cell growth.Endocrine · 1995Article
- Evidence for an interleukin 4-inducible immunoglobulin E uptake and transport mechanism in the intestine.The Journal of experimental medicine · 1994Article
- Control of rat natural killer cell-mediated allorecognition by a major histocompatibility complex region encoding nonclassical class I antigens.The Journal of experimental medicine · 1994Article
- Proliferative and cytokine responses to a major surface glycoprotein of Pneumocystis carinii.Infection and immunity · 1993Article
- The natural immune response to inhaled soluble protein antigens involves major histocompatibility complex (MHC) class I-restricted CD8+ T cell-mediated but MHC class II-restricted CD4+ T cell-dependent immune deviation resulting in selective suppression of immunoglobulin E production.The Journal of experimental medicine · 1993Article
- Glucocorticoids induce the expression of CD8 alpha chains on concanavalin A-activated rat CD4+ T cells: induction is inhibited by rat recombinant interleukin 4.The Journal of experimental medicine · 1992Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously described the isolation and sequencing of cDNA clones encoding rat interleukin-2 (IL-2) and interleukin-4 (IL-4). In the present study, we report the generation of stably transfected Chinese hamster ovary (CHO) cell lines which constitutively synthesize and secrete high levels of rat recombinant IL-2 (rIL-2) and IL-4 (rIL-4). The expression of the cytokine cDNA sequences is driven by the human cytomegalovirus promoter/enhancer within the respective pEE6. HCMV-GS vector constructs, following the successful transfection and isolation of methionine sulphoximine (MSX)-resistant CHO cell lines. Analyses of metabolically labelled CHO.rIL-2 and CHO.rIL-4 have been performed, in addition to studies which demonstrate certain biological properties of these recombinant cytokines including T-cell growth factor activity (rIL-2) and the ability to enhance expression of class II major histocompatibility complex (MHC) molecules on spleen cells (rIL-4). The availability of large quantities of these rat recombinant cytokines, conveniently produced by a mammalian cell line, will prove invaluable in future studies into the induction and regulation of immune responses in this species.
Indexed as
Identifiers
1551691PMC1384708W206883561What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.