ArticleHuman genetics1992
Characterization of centromere arrangements and test for random distribution in G0, G1, S, G2, G1, and early S' phase in human lymphocytes.
Article in Human genetics, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- Article
- CENP-A: A Histone H3 Variant with Key Roles in Centromere Architecture in Healthy and Diseased States.Results and problems in cell differentiation · 2022Article
- CENP-A Regulation and Cancer.Frontiers in cell and developmental biology · 2022Review
- Article
- Data-Driven Polymer Model for Mechanistic Exploration of Diploid Genome Organization.Biophysical journal · 2020Article
- Non-Canonical Functions of the Gamma-Tubulin Meshwork in the Regulation of the Nuclear Architecture.Cancers · 2020Review
- The Genomic Health of Human Pluripotent Stem Cells: Genomic Instability and the Consequences on Nuclear Organization.Frontiers in genetics · 2018Review
- Article
- Nucleolar aggresomes mediate release of pericentric heterochromatin and nuclear destruction of genotoxically treated cancer cells.Nucleus (Austin, Tex.) · 2017Article
- Population-based 3D genome structure analysis reveals driving forces in spatial genome organization.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Transcription and ncRNAs: at the cent(rome)re of kinetochore assembly and maintenance.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Review
- A role for the CAL1-partner Modulo in centromere integrity and accurate chromosome segregation in Drosophila.PloS one · 2012Article
- Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy.PloS one · 2011Article
- Quantitative analysis of cell nucleus organisation.PLoS computational biology · 2007Review
- Common themes and cell type specific variations of higher order chromatin arrangements in the mouse.BMC cell biology · 2005Article
- Article
- Nuclear organization of centromeric domains is not perturbed by inhibition of histone deacetylases.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Article
- Three-dimensional arrangements of centromeres and telomeres in nuclei of human and murine lymphocytes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Article
- Higher levels of organization in the interphase nucleus of cycling and differentiated cells.Microbiology and molecular biology reviews : MMBR · 2000Review
- Non-Rabl patterns of centromere and telomere distribution in the interphase nuclei of plant cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The arrangement of centromeres, cluster formation and association with the nucleolus and the nuclear membrane were characterized in human lymphocytes during the course of interphase in a cell-phase-dependent manner. We evaluated 3,893 cell nuclei categorized by five parameters. The centromeres were visualized by means of indirect immunofluorescent labeling with anti-centromere antibodies (ACA) contained in serum of patients with CREST syndrome. The cell nuclei were classified as G0, G1, S, G2, G1' and early S' phase by comparing microscopically identified groups of cell nuclei with flow cytometric determination of cell cycle stage of synchronized and unsynchronized lymphocyte cell cultures. Based on a discrimination analysis, a program was devised that calculated the probability for any cell nucleus belonging to the G0, G1, S, G2, G1' and early S' phase using only two microscopic parameters. Various characteristics were determined in the G0, S, and G2 stages. A transition stage to S phase within G1 was detected. This stage shows centromere arrangements not repeated in later cell cycles and which develop from the dissolution of centromere clusters in the periphery of the nucleus during G0 and G1. S phase exhibits various non-random centromere arrangements and associations of centromeres with the nucleolus. G1' and early S' phase of the second cell cycle display no characteristic centromere arrangement. The duplication of centromeres in G2 is asynchronous in two phases. For all cell phases a test for random distribution of the centromeres in the cell nucleus was performed. There is a distinct tendency for centromeres to be in a peripheral position during G0 and G1; this tendency becomes weaker in S phase. Although the visual impression is a seemingly random distribution of centromeres in G2 and G1', statistical analysis still demonstrates a significant deviation from random distribution in favor of a peripheral location. Only the early S phase of the second cell cycle shows no significant deviation from a random distribution.
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