ArticleBiophysical journal2005
Cholesterol depletion suppresses the translational diffusion of class II major histocompatibility complex proteins in the plasma membrane.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 119 citations in OpenAlex.
- T cell decision-making decodes the dynamic antigenic landscape.Frontiers in immunology · 2026Review
- Syntaxin clusters and cholesterol affect the mobility of Syntaxin1a.Biophysical journal · 2025Article
- Statin-induced increase in actin polymerization modulates GPCR dynamics and compartmentalization.Biophysical journal · 2023Article
- Remodeling of yeast vacuole membrane lipidomes from the log (one phase) to stationary stage (two phases).Biophysical journal · 2023Article
- Heterogeneous nanoscopic lipid diffusion in the live cell membrane and its dependency on cholesterol.Biophysical journal · 2022Article
- Membranes regulate biomolecular condensates.Nature cell biology · 2022Article
- Efficient Cholesterol Transport in Dendritic Cells Defines Optimal Exogenous Antigen Presentation and Toxoplasma gondii Proliferation.Frontiers in cell and developmental biology · 2022Article
- Motional dynamics of single Patched1 molecules in cilia are controlled by Hedgehog and cholesterol.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Mechanosensing in the immune response.Seminars in cell & developmental biology · 2017Review
- Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane.Molecular biology of the cell · 2016Article
- Changes in membrane sphingolipid composition modulate dynamics and adhesion of integrin nanoclusters.Scientific reports · 2016Article
- Lateral diffusion, function, and expression of the slow channel congenital myasthenia syndrome αC418W nicotinic receptor mutation with changes in lipid raft components.The Journal of biological chemistry · 2015Article
- A high fat diet containing saturated but not unsaturated fatty acids enhances T cell receptor clustering on the nanoscale.Prostaglandins, leukotrienes, and essential fatty acids · 2015Article
- Get your kICS by measuring membrane protein dynamics.Biophysical journal · 2015Article
- Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol.PLoS neglected tropical diseases · 2014Article
- Coupling between endocytosis and sphingosine kinase 1 recruitment.Nature cell biology · 2014Article
- Cell-surface translational dynamics of nicotinic acetylcholine receptors.Frontiers in synaptic neuroscience · 2014Review
- Transient cholesterol effects on nicotinic acetylcholine receptor cell-surface mobility.PloS one · 2014Article
- Organizing MHC Class II Presentation.Frontiers in immunology · 2014Review
- Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Glycosylphosphatidylinositol (GPI)-linked and native major histocompatibility complex class II I-E(k) were used as probes to determine the effect of varying cholesterol concentration on the mobility of proteins in the plasma membrane. These proteins were imaged in Chinese hamster ovary cells using single-molecule fluorescence microscopy. Observed diffusion coefficients of both native and GPI-linked I-E(k) proteins were found to depend on cholesterol concentration. As the cholesterol concentration decreases the diffusion coefficients decrease by up to a factor of 7 for native and 5 for GPI-linked I-E(k). At low cholesterol concentrations, after sphingomyelinase treatment, the diffusion coefficients are reduced by up to a factor of 60 for native and 190 for GPI-linked I-E(k). The effect is reversible on cholesterol reintroduction. Diffusion at all studied cholesterol concentrations, for both proteins, appears to be predominantly Brownian for time lags up to 2.5 s when imaged at 10 Hz. A decrease in diffusion coefficients is observed for other membrane proteins and lipid probes, DiIC12 and DiIC18. Fluorescence recovery after photobleaching measurements shows that the fraction of immobile lipid probe increases from 8 to approximately 40% after cholesterol extraction. These results are consistent with the previous work on cholesterol-phospholipid interactions. That is, cholesterol extraction destroys liquid cholesterol-phospholipid complexes, leaving solid-like high melting phospholipid domains that inhibit the lateral diffusion of membrane components.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.