ArticleBiophysical journal2005
A ring of negative charges in the intracellular vestibule of Kir2.1 channel modulates K+ permeation.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Activation of the CaPflugers Archiv : European journal of physiology · 2016Article
- Gating the glutamate gate of CLC-2 chloride channel by pore occupancy.The Journal of general physiology · 2016Article
- Mechanism for attenuated outward conductance induced by mutations in the cytoplasmic pore of Kir2.1 channels.Scientific reports · 2015Article
- The bundle crossing region is responsible for the inwardly rectifying internal spermine block of the Kir2.1 channel.Pflugers Archiv : European journal of physiology · 2014Article
- Control of KirBac3.1 potassium channel gating at the interface between cytoplasmic domains.The Journal of biological chemistry · 2014Article
- Revisiting inward rectification: K ions permeate through Kir2.1 channels during high-affinity block by spermidine.The Journal of general physiology · 2012Article
- Locale and chemistry of spermine binding in the archetypal inward rectifier Kir2.1.The Journal of general physiology · 2010Article
- Changes in negative charge at the luminal mouth of the pore alter ion handling and gating in the cardiac ryanodine-receptor.Biophysical journal · 2009Article
- Charges in the cytoplasmic pore control intrinsic inward rectification and single-channel properties in Kir1.1 and Kir2.1 channels.The Journal of membrane biology · 2007Article
- Functional roles of charged amino acid residues on the wall of the cytoplasmic pore of Kir2.1.The Journal of general physiology · 2006Article
- Differential polyamine sensitivity in inwardly rectifying Kir2 potassium channels.The Journal of physiology · 2006Article
- Electrostatics in the cytoplasmic pore produce intrinsic inward rectification in kir2.1 channels.The Journal of general physiology · 2005Article
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3 authors.
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Abstract
The glutamate at site 224 of a Kir2.1 channel plays an important role in K+ permeation. The single-channel inward current flickers with reduced conductance in an E224G mutant. We show that open-channel fluctuations can also be observed in E224C, E224K, and E224Q mutants. Yet, open-channel fluctuations were not observed in either the wild-type or an E224D mutant. Introducing a negatively charged methanethiosulfonate reagent to the E224C mutant irreversibly increased channel conductance and eliminated open-channel fluctuations. These results suggest that although the negatively charged residue 224 is located at the internal vestibule, it is important for smooth inward K+ conduction. We identified a substate in the E224G mutant and showed that open-channel fluctuations are mainly attributed to rapid transitions between the substate and the main state. Also, we characterized the voltage- and ion-dependence of the substate kinetics. The open-channel fluctuations decreased in internal NH4+ or Tl+ as compared to internal K+. These results suggest that NH4+ and Tl+ gate the E224G mutant in a more stable state. Based on an ion-conduction model, we propose that the appearance of the substate in the E224G mutant is due to changes of ion gating in association with variations of ion-ion interaction in the permeation pathway.
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