ArticleGenetics1992
Nonsense suppression of the major rhodopsin gene of Drosophila.
Article in Genetics, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Applications of genetic code expansion technology in eukaryotes.Protein & cell · 2024Review
- Olfactory receptor pseudo-pseudogenes.Nature · 2016Article
- Rhodopsin coexpression in UV photoreceptors of Aedes aegypti and Anopheles gambiae mosquitoes.The Journal of experimental biology · 2014Article
- Expanding the genetic code of Drosophila melanogaster.Nature chemical biology · 2012Article
- A role for the cytoplasmic DEAD box helicase Dbp21E2 in rhodopsin maturation and photoreceptor viability.Journal of neurogenetics · 2012Article
- Evidence of abundant stop codon readthrough in Drosophila and other metazoa.Genome research · 2011Article
- Article
- Molecular and genetic characterization of the interactions between the Drosophila stoned-B protein and DAP-160 (intersectin).The Biochemical journal · 2005Article
- Article
- Limited role of developmental programmed cell death pathways in Drosophila norpA retinal degeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Article
- Mutations in eukaryotic release factors 1 and 3 act as general nonsense suppressors in Drosophila.Genetics · 2003Article
- Isolation of Su(var)3-7 mutations by homologous recombination in Drosophila melanogaster.Genetics · 2002Article
- Invertebrate synapsins: a single gene codes for several isoforms in Drosophila.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Expression of a Drosophila melanogaster amber suppressor tRNA(Ser) in Caenorhabditis elegans.Molecular & general genetics : MGG · 1993Article
- Transcript identification in the optomotor-blind locus of Drosophila melanogaster by intragenic recombination mapping and PCR-aided sequence analysis of lethal point mutations.Molecular & general genetics : MGG · 1993Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We placed UAA, UAG and UGA nonsense mutations at two leucine codons, Leu205 and Leu309, in Drosophila's major rhodopsin gene, ninaE, by site-directed mutagenesis, and then created the corresponding mutants by P element-mediated transformation of a ninaE deficiency strain. In the absence of a genetic suppressor, flies harboring any of the nonsense mutations at the 309 site, but not the 205 site, show increased rhodopsin activity. Additionally, all flies with nonsense mutations at either site have better rhabdomere structure than does the ninaE deficiency strain. Construction and analysis of a 3'-deletion mutant of ninaE indicates that translational readthrough accounts for the extra photoreceptor activity of the ninaE309 alleles and that truncated opsins are responsible for the improved rhabdomere structure. The presence of leucine-inserting tRNA nonsense suppressors DtLa Su+ and DtLb Su+ in the mutant strains produced a small increase (less than 0.04%) in functional rhodopsin. The opal (UGA) suppressor derived from the DtLa tRNA gene is more efficient than the amber (UAG) or opal suppressor derived from the DtLb gene, and both DtLa and DtLb derived suppressors are more efficient at site 205 than 309.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.