ArticleNucleic acids research2004
Acinetobacter sp. ADP1: an ideal model organism for genetic analysis and genome engineering.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed.
- Mechanistic basis of LolDF-mediated lipoprotein transport in Acinetobacter.Nature communications · 2026Article
- Conformational hijacking of lipoprotein transporter LolDF enables precision antimicrobial activity against Acinetobacter baumannii.Nature communications · 2026Article
- Protein overabundance is driven by growth robustness.Science advances · 2026Article
- Expression of a phage RecET recombinase system improves detection of shorter DNA fragments by anmicroPublication biology · 2026Article
- History of the taxonomy ofInternational journal of systematic and evolutionary microbiology · 2025Article
- Conversion and upgrading of syringate by Acinetobacter baylyi ADP1.Microbial cell factories · 2025Article
- Improved DNA binding to a type IV minor pilin increases natural transformation.Nucleic acids research · 2025Article
- Exploring the Multifaceted GenusJournal of microbiology and biotechnology · 2025Review
- The elimination of two restriction enzyme genes allows for electroporation-based transformation and CRISPR-Cas9-based base editing in the non-competent Gram-negative bacteriumApplied and environmental microbiology · 2024Article
- Carbon-wise utilization of lignin-related compounds by synergistically employing anaerobic and aerobic bacteria.Biotechnology for biofuels and bioproducts · 2024Article
- DNA binding is rate-limiting for natural transformation.bioRxiv : the preprint server for biology · 2024Article
- Enhanced upgrading of lignocellulosic substrates by coculture of Saccharomyces cerevisiae and Acinetobacter baylyi ADP1.Biotechnology for biofuels and bioproducts · 2024Article
- AlmA involved in the long-chainApplied and environmental microbiology · 2024Article
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- Genome-scale analysis of essential gene knockout mutants to identify an antibiotic target process.Antimicrobial agents and chemotherapy · 2023Article
- A comprehensive genomic analysis provides insights on the high environmental adaptability ofFrontiers in microbiology · 2023Article
- Evaluation of Antibiotic Resistance andIranian journal of public health · 2022Article
- Engineering cell morphology by CRISPR interference in Acinetobacter baylyi ADP1.Microbial biotechnology · 2022Article
- Subcellular localization of Type VI secretion system assembly in response to cell-cell contact.The EMBO journal · 2022Article
32 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Acinetobacter sp. strain ADP1 is a naturally transformable gram-negative bacterium with simple culture requirements, a prototrophic metabolism and a compact genome of 3.7 Mb which has recently been sequenced. Wild-type ADP1 can be genetically manipulated by the direct addition of linear DNA constructs to log-phase cultures. This makes it an ideal organism for the automation of complex strain construction. Here, we demonstrate the flexibility and versatility of ADP1 as a genetic model through the construction of a broad variety of mutants. These include marked and unmarked insertions and deletions, complementary replacements, chromosomal expression tags and complex combinations thereof. In the process of these constructions, we demonstrate that ADP1 can effectively express a wide variety of foreign genes including antibiotic resistance cassettes, essential metabolic genes, negatively selectable catabolic genes and even intact operons from highly divergent bacteria. All of the described mutations were achieved by the same process of splicing PCR, direct transformation of growing cultures and plating on selective media. The simplicity of these tools make genetic analysis and engineering with Acinetobacter ADP1 accessible to laboratories with minimal microbial genetics expertise and very little equipment. They are also compatible with complete automation of genetic analysis and engineering protocols.
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