ArticleGenetics2004
Mutations in SIN4 and RGR1 cause constitutive expression of MAL structural genes in Saccharomyces cerevisiae.
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 33 citations in OpenAlex.
- Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.PLoS genetics · 2021Article
- Essential role of MED1 in the transcriptional regulation of ER-dependent oncogenic miRNAs in breast cancer.Scientific reports · 2018Article
- The Arabidopsis mediator complex subunits MED16, MED14, and MED2 regulate mediator and RNA polymerase II recruitment to CBF-responsive cold-regulated genes.The Plant cell · 2014Article
- Facilitated assembly of the preinitiation complex by separated tail and head/middle modules of the mediator.Journal of molecular biology · 2012Article
- Med5(Nut1) and Med17(Srb4) are direct targets of mediator histone H4 tail interactions.PloS one · 2012Article
- Histone modifications influence mediator interactions with chromatin.Nucleic acids research · 2011Article
- Hsp90 cochaperone Aha1 is a negative regulator of the Saccharomyces MAL activator and acts early in the chaperone activation pathway.The Journal of biological chemistry · 2010Article
- Artificial recruitment of mediator by the DNA-binding domain of Adr1 overcomes glucose repression of ADH2 expression.Molecular and cellular biology · 2008Article
- Analysis of transcriptional activation at a distance in Saccharomyces cerevisiae.Molecular and cellular biology · 2007Article
- Fermentation of high concentrations of maltose by Saccharomyces cerevisiae is limited by the COMPASS methylation complex.Applied and environmental microbiology · 2006Article
- A functional module of yeast mediator that governs the dynamic range of heat-shock gene expression.Genetics · 2006Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Transcription of the Saccharomyces MAL structural genes is induced 40-fold by maltose and requires the MAL-activator and maltose permease. To identify additional players involved in regulating MAL gene expression, we carried out a genetic selection for MAL constitutive mutants. Strain CMY4000 containing MAL1 and integrated copies of MAL61promoter-HIS3 and MAL61promoter-lacZ reporter genes was used to select constitutive mutants. The 29 recessive mutants fall into at least three complementation groups. Group 1 and group 2 mutants exhibit pleiotropic phenotypes and represent alleles of Mediator component genes RGR1 and SIN4, respectively. The rgr1 and sin4 constitutive phenotype does not require either the MAL-activator or maltose permease, indicating that Mediator represses MAL basal expression. Further genetic analysis demonstrates that RGR1 and SIN4 work in a common pathway and each component of the Mediator Sin4 module plays a distinct role in regulating MAL gene expression. Additionally, the Swi/Snf chromatin-remodeling complex is required for full induction, suggesting a role for chromatin remodeling in the regulation of MAL gene expression. A sin4Delta mutation is unable to suppress the defects in MAL gene expression resulting from loss of the Swi/Snf complex component Snf2p. The role of the Mediator in MAL gene regulation is discussed.
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