ArticleGenetics2004
Construction and evaluation of cDNA libraries for large-scale expressed sequence tag sequencing in wheat (Triticum aestivum L.).
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Potential role of the regulatoryFrontiers in plant science · 2023Article
- Genetics of destemming in pepper: A step towards mechanical harvesting.Frontiers in genetics · 2023Article
- Dissection of Z-disc myopalladin gene network involved in the development of restrictive cardiomyopathy using system genetics approach.World journal of cardiology · 2017Article
- De Novo Assembled Wheat Transcriptomes Delineate Differentially Expressed Host Genes in Response to Leaf Rust Infection.PloS one · 2016Article
- De novo assembly and characterization of transcriptome using Illumina paired-end sequencing and identification of CesA gene in ramie (Boehmeria nivea L. Gaud).BMC genomics · 2013Article
- Ginger and turmeric expressed sequence tags identify signature genes for rhizome identity and development and the biosynthesis of curcuminoids, gingerols and terpenoids.BMC plant biology · 2013Article
- Comprehensive functional analyses of expressed sequence tags in common wheat (Triticum aestivum).DNA research : an international journal for rapid publication of reports on genes and genomes · 2012Article
- Development of expressed sequence tag resources for Vanda Mimi Palmer and data mining for EST-SSR.Molecular biology reports · 2011Article
- The spectrum of low molecular weight alpha-amylase/protease inhibitor genes expressed in the US bread wheat cultivar Butte 86.BMC research notes · 2011Article
- Conserved globulin gene across eight grass genomes identify fundamental units of the loci encoding seed storage proteins.Functional & integrative genomics · 2010Article
- Article
- Genome-wide analysis of the rice and Arabidopsis non-specific lipid transfer protein (nsLtp) gene families and identification of wheat nsLtp genes by EST data mining.BMC genomics · 2008Article
- Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana.Genome research · 2007Article
- Mapping translocation breakpoints using a wheat microarray.Nucleic acids research · 2007Article
- Resistance gene analogues of wheat: molecular genetic analysis of ESTs.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Wheat EST resources for functional genomics of abiotic stress.BMC genomics · 2006Article
- Transcriptome analysis of salinity stress responses in common wheat using a 22k oligo-DNA microarray.Functional & integrative genomics · 2006Article
- Analysis of the wheat endosperm transcriptome.Journal of applied genetics · 2006Article
- Article
Corrections and comments
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Authors and funding
43 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A total of 37 original cDNA libraries and 9 derivative libraries enriched for rare sequences were produced from Chinese Spring wheat (Triticum aestivum L.), five other hexaploid wheat genotypes (Cheyenne, Brevor, TAM W101, BH1146, Butte 86), tetraploid durum wheat (T. turgidum L.), diploid wheat (T. monococcum L.), and two other diploid members of the grass tribe Triticeae (Aegilops speltoides Tausch and Secale cereale L.). The emphasis in the choice of plant materials for library construction was reproductive development subjected to environmental factors that ultimately affect grain quality and yield, but roots and other tissues were also included. Partial cDNA expressed sequence tags (ESTs) were examined by various measures to assess the quality of these libraries. All ESTs were processed to remove cloning system sequences and contaminants and then assembled using CAP3. Following these processing steps, this assembly yielded 101,107 sequences derived from 89,043 clones, which defined 16,740 contigs and 33,213 singletons, a total of 49,953 "unigenes." Analysis of the distribution of these unigenes among the libraries led to the conclusion that the enrichment methods were effective in reducing the most abundant unigenes and to the observation that the most diverse libraries were from tissues exposed to environmental stresses including heat, drought, salinity, or low temperature.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.