Evidence map›Paper›PMID 15514037›Full record

ArticleGenetics2004

Development of an expressed sequence tag (EST) resource for wheat (Triticum aestivum L.): EST generation, unigene analysis, probe selection and bioinformatics for a 16,000-locus bin-delineated map.

G R Lazo, S Chao, D D Hummel, H Edwards, C C Crossman, N Lui, D E Matthews, V L Carollo, D L Hane, F M You and 31 more

Abstract read
In one paragraph

Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Utilization of deletion bins to anchor and order sequences along the wheat 7B chromosome.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. dbWFA: a web-based database for functional annotation of Triticum aestivum transcripts.Database : the journal of biological databases and curation · 2013
    Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Pm37, a new broadly effective powdery mildew resistance gene from Triticum timopheevii.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

41 authors.

G R LazoU.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Western Regional Research Center, Albany, California 94710-1105, USA.
S Chao
D D Hummel
H Edwards
C C Crossman
N Lui
D E Matthews
V L Carollo
D L Hane
F M You
G E Butler
R E Miller
T J Close
J H Peng
N L V Lapitan
J P Gustafson
L L Qi
B Echalier
B S Gill
M Dilbirligi
H S Randhawa
K S Gill
R A Greene
M E Sorrells
E D Akhunov
J Dvorák
A M Linkiewicz
J Dubcovsky
K G Hossain
V Kalavacharla
S F Kianian
A A Mahmoud
Miftahudin
X-F Ma
E J Conley
J A Anderson
M S Pathan
H T Nguyen
P E McGuire
C O Qualset
O D Anderson

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This report describes the rationale, approaches, organization, and resource development leading to a large-scale deletion bin map of the hexaploid (2n = 6x = 42) wheat genome (Triticum aestivum L.). Accompanying reports in this issue detail results from chromosome bin-mapping of expressed sequence tags (ESTs) representing genes onto the seven homoeologous chromosome groups and a global analysis of the entire mapped wheat EST data set. Among the resources developed were the first extensive public wheat EST collection (113,220 ESTs). Described are protocols for sequencing, sequence processing, EST nomenclature, and the assembly of ESTs into contigs. These contigs plus singletons (unassembled ESTs) were used for selection of distinct sequence motif unigenes. Selected ESTs were rearrayed, validated by 5' and 3' sequencing, and amplified for probing a series of wheat aneuploid and deletion stocks. Images and data for all Southern hybridizations were deposited in databases and were used by the coordinators for each of the seven homoeologous chromosome groups to validate the mapping results. Results from this project have established the foundation for future developments in wheat genomics.

Indexed as

Chromosome MappingComputational BiologyContig MappingGene DeletionBlotting, SouthernDNA ProbesExpressed Sequence TagsGene LibraryTriticumDNA Probes

Identifiers

PMID15514037
PMCPMC1448819

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.