ArticleThe Journal of physiology2005
Relations between substrate affinities and charge equilibration rates in the rat GABA cotransporter GAT1.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Structural characterization reveals substrate recognition by the taurine transporter TauT.Cell discovery · 2025Article
- Functional consequences of sulfhydryl modification of the γ-aminobutyric acid transporter 1 at a single solvent-exposed cysteine residue.The Journal of membrane biology · 2012Article
- An inverse relationship links temperature and substrate apparent affinity in the ion-coupled cotransporters rGAT1 and KAAT1.International journal of molecular sciences · 2012Article
- Temperature effects on the kinetic properties of the rabbit intestinal oligopeptide cotransporter PepT1.Pflugers Archiv : European journal of physiology · 2012Article
- Inhibitors of the gamma-aminobutyric acid transporter 1 (GAT1) do not reveal a channel mode of conduction.Neurochemistry international · 2009Article
- GABA transporter lysine 448: a key residue for tricyclic antidepressants interaction.Cellular and molecular life sciences : CMLS · 2009Article
- Turnover rate of the gamma-aminobutyric acid transporter GAT1.The Journal of membrane biology · 2007Article
- Molecular interactions between dipeptides, drugs and the human intestinal H+ -oligopeptide cotransporter hPEPT1.The Journal of physiology · 2006Article
- Role of the conserved glutamine 291 in the rat gamma-aminobutyric acid transporter rGAT-1.Cellular and molecular life sciences : CMLS · 2006Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The relations between apparent affinity for substrates and operating rates have been investigated by two-electrode voltage clamp in the GABA transporter rGAT1 expressed in Xenopus oocytes. We have measured the transport current induced by the presence of GABA, as well as the charge equilibration rate in the absence of the neurotransmitter, in various experimental conditions known to affect the transporter characteristics. The apparent affinities for GABA and for Na(+) were also determined in the same conditions. Two pharmacological actions and three mutated isoforms have been examined. In all cases significant correlations were found between the charge equilibration rates and apparent affinities for both substrates. In particular in the transport process, the apparent affinity for GABA appears to be inversely related to the sum of the unidirectional charge equilibration rates (alpha+beta), while the Na(+) apparent affinity is directly related to their ratio (beta/alpha). Together these observations suggest a kinetic basis for GABA affinity with higher turnover rates resulting in lower affinity, and indicate that an efficient uptake requires a compromise between these two parameters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.