ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2004
A PCR based B-genome-specific marker in Brassica species.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Dissection of B subgenome of Brassica juncea through monosomic addition lines in Brassica napus: phenomic, cytological and genotypic insights.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Development of Brassica rapa-nigra monosomic alien addition lines: cytology, genetics, and morphology.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Transcriptional Interactions of Single B-Subgenome Chromosome with C-Subgenome inPlants (Basel, Switzerland) · 2023Article
- Production and cytology of Brassica autoallohexaploids with two and four copies of two subgenomes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Subgenome Discrimination inCells · 2021Article
- Genomic insights into the origin, domestication and diversification of Brassica juncea.Nature genetics · 2021Article
- Gene Flow Risks From Transgenic Herbicide-Tolerant Crops to Their Wild Relatives Can Be Mitigated by Utilizing Alien Chromosomes.Frontiers in plant science · 2021Article
- Investigating genetic relationship ofMolecular breeding : new strategies in plant improvement · 2021Article
- A high-contiguity Brassica nigra genome localizes active centromeres and defines the ancestral Brassica genome.Nature plants · 2020Article
- Development of a DNA Barcoding-Like Approach to Detect Mustard Allergens in Wheat Flours.Genes · 2019Article
- Development of Brassica oleracea-nigra monosomic alien addition lines: genotypic, cytological and morphological analyses.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Cytogenetic and Molecular Characterization of B-Genome Introgression Lines of Brassica napus L.G3 (Bethesda, Md.) · 2017Article
- A multiplex PCR for rapid identification ofPlant methods · 2017Article
- Distinct subgenome stabilities in synthesized Brassica allohexaploids.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Cloning of TTG1 gene and PCR identification of genomes A, B and C in Brassica species.Genetica · 2014Article
- Seed coat microsculpturing is related to genomic components in wild Brassica juncea and Sinapis arvensis.PloS one · 2013Article
- Different genome-specific chromosome stabilities in synthetic Brassica allohexaploids revealed by wide crosses with Orychophragmus.Annals of botany · 2009Article
- Cytogenetics and genome analysis in Brassica crops.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Review
- Molecular cytogenetic analysis of Brassica rapa-Brassica oleracea var. alboglabra monosomic addition lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
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Authors and funding
4 authors.
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Abstract
Previous hybridisation studies showed that the repetitive DNA sequence pBNBH35 from Brassica nigra (genome BB, 2n=16) bound specifically to the B-genome and not to the A- or C-genomes of Brassica species. We amplified a sub-fragment of pBNBH35 from B. nigra by PCR, cloned and sequenced this sub-fragment, and confirmed that it was a 329-bp sub-fragment of pBNBH35. PCR and hybridisation techniques were used to confirm that the pBNBH35 sub-fragment was Brassica B-genome-specific. Fluorescence in situ hybridisation (FISH) in B. nigra, B. juncea (AABB, 2n=36) and B. napus (AACC, 2n=38) showed that the pBNBH35 sub-fragment was present on all eight Brassica B-genome chromosomes and absent from the A- and C-genome chromosomes. The pBNBH35 repeat was localised to the centromeric region of each B-genome chromosome. FISH clearly distinguished the B-genome chromosomes from the A-genome chromosomes in the amphidiploid species B. juncea. This is the first known report of a B-genome repetitive marker that is present on all B-genome chromosomes. It will be a useful tool for the detection of B chromosomes in interspecific hybrids and may prove useful for phylogenetic studies in Brassica species.
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