ArticleThe EMBO journal2004
LAP2alpha and BAF collaborate to organize the Moloney murine leukemia virus preintegration complex.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Alternative splicing and co-option of transposable elements: the case of TMPO/LAP2α and ZNF451 in mammals.Bioinformatics (Oxford, England) · 2015Article
- Role of host-encoded proteins in restriction of retroviral integration.Frontiers in microbiology · 2012Article
- Molecular characterization of the host defense activity of the barrier to autointegration factor against vaccinia virus.Journal of virology · 2011Article
- Review
- The Gag cleavage product, p12, is a functional constituent of the murine leukemia virus pre-integration complex.PLoS pathogens · 2010Article
- Transcription factor YY1 interacts with retroviral integrases and facilitates integration of moloney murine leukemia virus cDNA into the host chromosomes.Journal of virology · 2010Article
- Functional disruption of the moloney murine leukemia virus preintegration complex by vaccinia-related kinases.The Journal of biological chemistry · 2010Article
- Article
- Immunity of replicating Mu to self-integration: a novel mechanism employing MuB protein.Mobile DNA · 2010Article
- Barrier-to-autointegration factor (BAF) condenses DNA by looping.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The host cell sulfonation pathway contributes to retroviral infection at a step coincident with provirus establishment.PLoS pathogens · 2008Article
- Article
- The LEM domain proteins emerin and LAP2alpha are dispensable for human immunodeficiency virus type 1 and murine leukemia virus infections.Journal of virology · 2008Article
- Nuclear lamins: major factors in the structural organization and function of the nucleus and chromatin.Genes & development · 2008Review
- Mouse mammary tumor virus integration site selection in human and mouse genomes.Journal of virology · 2008Article
- The road to chromatin - nuclear entry of retroviruses.Nature reviews. Microbiology · 2007Review
- Wild-type levels of human immunodeficiency virus type 1 infectivity in the absence of cellular emerin protein.Journal of virology · 2007Article
- Retrotransposon suicide: formation of Ty1 circles and autointegration via a central DNA flap.Journal of virology · 2006Article
- Mechanisms and inhibition of HIV integration.Drug discovery today. Disease mechanisms · 2006Article
- Barrier-to-autointegration factor phosphorylation on Ser-4 regulates emerin binding to lamin A in vitro and emerin localization in vivo.Molecular biology of the cell · 2006Article
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Authors and funding
3 authors.
Funding
Abstract
Integration of viral DNA into the host genome is an essential step in retroviral replication. The viral DNA made by reverse transcription is a component of the preintegration complex (PIC) that also contains the viral integrase protein, the enzyme that integrates the viral DNA. Several other viral and cellular proteins are present in the PIC, but their functional roles are less well established. Barrier-to-autointegration factor (BAF) is a cellular protein component of the PIC that blocks autointegration of the viral DNA and stimulates intermolecular integration. In uninfected cells, BAF interacts with members of the LEM family of inner nuclear membrane and nucleoplasmic proteins. Here, we demonstrate that one of the LEM proteins, lamina-associated polypeptide 2alpha (LAP2alpha), is a component of the PIC. LAP2alpha stabilizes the association of BAF with the PIC to stimulate intermolecular integration and suppress autointegration. To further understand the role of LAP2alpha, we established LAP2alpha-knockdown cell lines. Depletion of LAP2alpha significantly inhibited viral replication. Our results demonstrate a critical contribution of LAP2alpha to the nucleoprotein organization of the PIC and to viral replication.
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