ArticleMolecular and cellular biology2004
NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- High-resolution binding data of TFIID and cofactors show promoter-specific differences in vivo.Research square · 2026Article
- High-resolution binding data of TFIID and cofactors show promoter-specific differencesbioRxiv : the preprint server for biology · 2025Article
- Analyzing the Dynamics of Single TBP-DNA-NC2 Complexes Using Hidden Markov Models.Biophysical journal · 2018Article
- Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.Molecular cell · 2017Article
- Molecular Mechanism of Mot1, a TATA-binding Protein (TBP)-DNA Dissociating Enzyme.The Journal of biological chemistry · 2016Article
- Article
- Structure and mechanism of the Swi2/Snf2 remodeller Mot1 in complex with its substrate TBP.Nature · 2011Article
- The basal initiation machinery: beyond the general transcription factors.Current opinion in cell biology · 2009Review
- Regulation of nuclear import and export of negative cofactor 2.The Journal of biological chemistry · 2009Article
- TATA-binding protein variants that bypass the requirement for Mot1 in vivo.The Journal of biological chemistry · 2009Article
- Maneuver at the transcription start site: Mot1p and NC2 navigate TFIID/TBP to specific core promoter elements.Epigenetics · 2009Article
- The dual control of TFIIB recruitment by NC2 is gene specific.Nucleic acids research · 2008Article
- Global distribution of negative cofactor 2 subunit-alpha on human promoters.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A TATA binding protein regulatory network that governs transcription complex assembly.Genome biology · 2007Article
- MAL and ternary complex factor use different mechanisms to contact a common surface on the serum response factor DNA-binding domain.Molecular and cellular biology · 2006Article
- Interaction of protein inhibitor of activated STAT (PIAS) proteins with the TATA-binding protein, TBP.The Journal of biological chemistry · 2006Article
- Mutational analysis of BTAF1-TBP interaction: BTAF1 can rescue DNA-binding defective TBP mutants.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptional activity of the TATA-binding protein (TBP) is controlled by a variety of proteins. The BTAF1 protein (formerly known as TAF(II)170/TAF-172 and the human ortholog of Saccharomyces cerevisiae Mot1p) and the NC2 complex composed of NC2alpha (DRAP1) and NC2beta (Dr1) are able to bind to TBP directly and regulate RNA polymerase II transcription both positively and negatively. Here, we present evidence that the NC2alpha subunit interacts with BTAF1. In contrast, the NC2beta subunit is not able to associate with BTAF1 and seems to interfere with the BTAF1-TBP interaction. Addition of NC2alpha or the NC2 complex can stimulate the ability of BTAF1 to interact with TBP. This function is dependent on the presence of ATP in cell extracts but does not involve the ATPase activity of BTAF1 nor phosphorylation of NC2alpha. Together, our results constitute the first evidence of the physical cooperation between BTAF1 and NC2alpha in TBP regulation and provide a framework to understand transcription functions of NC2alpha and NC2beta in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.