ArticleMolecular and cellular biology2004
Requirement of the Mre11 complex and exonuclease 1 for activation of the Mec1 signaling pathway.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 111 citations in OpenAlex.
- Slx4 and Fun30/SMARCAD1 coordinate S-phase checkpoint regulation and replication fork protection in response to Top1-DNA crosslinks.Nucleic acids research · 2026Article
- ATR-binding lncRNA ScaRNA2 promotes cancer resistance through facilitating efficient DNA end resection during homologous recombination repair.Journal of experimental & clinical cancer research : CR · 2023Article
- Quantitative mechanisms of DNA damage sensing and signaling.Current genetics · 2020Review
- Spatial separation between replisome- and template-induced replication stress signaling.The EMBO journal · 2018Article
- Genome maintenance functions of the INO80 chromatin remodeller.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Two separate pathways regulate protein stability of ATM/ATR-related protein kinases Mec1 and Tel1 in budding yeast.PLoS genetics · 2017Article
- The RNA binding protein Npl3 promotes resection of DNA double-strand breaks by regulating the levels of Exo1.Nucleic acids research · 2017Article
- The Intra-S Checkpoint Responses to DNA Damage.Genes · 2017Review
- Hsp90 induces increased genomic instability toward DNA-damaging agents by tuning down RAD53 transcription.Molecular biology of the cell · 2016Article
- DNA Replication Stress Phosphoproteome Profiles Reveal Novel Functional Phosphorylation Sites on Xrs2 in Saccharomyces cerevisiae.Genetics · 2016Article
- EXO1 is critical for embryogenesis and the DNA damage response in mice with a hypomorphic Nbs1 allele.Nucleic acids research · 2015Article
- Biochemical mechanism of DSB end resection and its regulation.DNA repair · 2015Review
- Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex.Genes & development · 2015Article
- Inhibition of telomere recombination by inactivation of KEOPS subunit Cgi121 promotes cell longevity.PLoS genetics · 2015Article
- Functional interplay between ATM/ATR-mediated DNA damage response and DNA repair pathways in oxidative stress.Cellular and molecular life sciences : CMLS · 2014Review
- Ddc2 mediates Mec1 activation through a Ddc1- or Dpb11-independent mechanism.PLoS genetics · 2014Article
- To trim or not to trim: progression and control of DSB end resection.Cell cycle (Georgetown, Tex.) · 2013Article
- Epigenetic silencing mediates mitochondria stress-induced longevity.Cell metabolism · 2013Article
- Dissection of DNA damage responses using multiconditional genetic interaction maps.Molecular cell · 2013Article
- Initiation of DNA damage responses through XPG-related nucleases.The EMBO journal · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The large protein kinases, ataxia-telangiectasia mutated (ATM) and ATM-Rad3-related (ATR), orchestrate DNA damage checkpoint pathways. In budding yeast, ATM and ATR homologs are encoded by TEL1 and MEC1, respectively. The Mre11 complex consists of two highly related proteins, Mre11 and Rad50, and a third protein, Xrs2 in budding yeast or Nbs1 in mammals. The Mre11 complex controls the ATM/Tel1 signaling pathway in response to double-strand break (DSB) induction. We show here that the Mre11 complex functions together with exonuclease 1 (Exo1) in activation of the Mec1 signaling pathway after DNA damage and replication block. Mec1 controls the checkpoint responses following UV irradiation as well as DSB induction. Correspondingly, the Mre11 complex and Exo1 play an overlapping role in activation of DSB- and UV-induced checkpoints. The Mre11 complex and Exo1 collaborate in producing long single-stranded DNA (ssDNA) tails at DSB ends and promote Mec1 association with the DSBs. The Ddc1-Mec3-Rad17 complex associates with sites of DNA damage and modulates the Mec1 signaling pathway. However, Ddc1 association with DSBs does not require the function of the Mre11 complex and Exo1. Mec1 controls checkpoint responses to stalled DNA replication as well. Accordingly, the Mre11 complex and Exo1 contribute to activation of the replication checkpoint pathway. Our results provide a model in which the Mre11 complex and Exo1 cooperate in generating long ssDNA tracts and thereby facilitate Mec1 association with sites of DNA damage or replication block.
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