ArticleMolecular and cellular biology2004
Novel functional dissection of the localization-specific roles of budding yeast polo kinase Cdc5p.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Yeast centrosomes act as organizing centers to promote Polo kinase-mediated adaptation to persistent DNA damage.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Spo13/Meikin pathway confines the onset of gamete differentiation to meiosis II in yeast.The EMBO journal · 2022Article
- Article
- Cell-cycle phospho-regulation of the kinetochore.Current genetics · 2021Review
- Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA.PLoS genetics · 2020Article
- Review
- Budding YeastG3 (Bethesda, Md.) · 2018Article
- Functions and regulation of the Polo-like kinase Cdc5 in the absence and presence of DNA damage.Current genetics · 2018Review
- Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeasteLife · 2017Article
- Article
- The budding yeast Polo-like kinase localizes to distinct populations at centrosomes during mitosis.Molecular biology of the cell · 2017Article
- Cell cycle-dependent phosphorylation of Sec4p controls membrane deposition during cytokinesis.The Journal of cell biology · 2016Article
- Article
- The budding yeast Polo-like kinase Cdc5 is released from the nucleus during anaphase for timely mitotic exit.Cell cycle (Georgetown, Tex.) · 2014Article
- Cell cycle checkpoint regulators reach a zillion.Cell cycle (Georgetown, Tex.) · 2013Review
- Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method.Genome research · 2013Article
- A dynamical model of the spindle position checkpoint.Molecular systems biology · 2012Article
- Phosphorylation of Cdc5 regulates its accumulation.Cell division · 2011Article
- Independent modulation of the kinase and polo-box activities of Cdc5 protein unravels unique roles in the maintenance of genome stability.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization.Molecular biology of the cell · 2011Article
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9 authors.
Funding
Abstract
Budding yeast polo kinase Cdc5p localizes to the spindle pole body (SPB) and to the bud-neck and plays multiple roles during M-phase progression. To dissect localization-specific mitotic functions of Cdc5p, we tethered a localization-defective N-terminal kinase domain of Cdc5p (Cdc5pDeltaC) to the SPB or to the bud-neck with components specifically localizing to one of these sites and characterized these mutants in a cdc5Delta background. Characterization of a viable, SPB-localizing, CDC5DeltaC-CNM67 mutant revealed that it is defective in timely degradation of Swe1p, a negative regulator of Cdc28p. Loss of BFA1, a negative regulator of mitotic exit, rescued the lethality of a neck-localizing CDC5DeltaC-CDC12 or CDC5DeltaC-CDC3 mutant but yielded severe defects in cytokinesis. These data suggest that the SPB-associated Cdc5p activity is critical for both mitotic exit and cytokinesis, whereas the bud neck-localized Cdc5p is required for proper Swe1p regulation. Interestingly, a cdc5Delta bfa1Delta swe1Delta triple mutant is viable but grows slowly, whereas cdc5Delta cells bearing both CDC5DeltaC-CNM67 and CDC5DeltaC-CDC12 grow well with only a mild cell cycle delay. Thus, SPB- and the bud-neck-localized Cdc5p control most of the critical Cdc5p functions and downregulation of Bfa1p and Swe1p at the respective locations are two critical factors that require Cdc5p.
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