ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
Glutamate mediates acute glucose transport inhibition in hippocampal neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 135 citations in OpenAlex.
- Lactate metabolism and histone lactylation in the central nervous system disorders: impacts and molecular mechanisms.Journal of neuroinflammation · 2024Review
- Variable bioenergetic sensitivity of neurons and astrocytes to insulin and extracellular glucose.npj metabolic health and disease · 2024Article
- The potential mechanisms of lactate in mediating exercise-enhanced cognitive function: a dual role as an energy supply substrate and a signaling molecule.Nutrition & metabolism · 2022Review
- Intertwined ROS and Metabolic Signaling at the Neuron-Astrocyte Interface.Neurochemical research · 2021Article
- Aerobic Glycolysis in the Brain: Warburg and Crabtree Contra Pasteur.Neurochemical research · 2021Review
- Metabolic compartmentalization between astroglia and neurons in physiological and pathophysiological conditions of the neurovascular unit.Neuropathology : official journal of the Japanese Society of Neuropathology · 2020Review
- Age-related hyperinsulinemia leads to insulin resistance in neurons and cell-cycle-induced senescence.Nature neuroscience · 2019Article
- Quantitative in vivo imaging of neuronal glucose concentrations with a genetically encoded fluorescence lifetime sensor.Journal of neuroscience research · 2019Article
- Interferon-alpha-Induced Changes in NODDI Predispose to the Development of Fatigue.Neuroscience · 2019Article
- Article
- Dual action of L-Lactate on the activity of NR2B-containing NMDA receptors: from potentiation to neuroprotection.Scientific reports · 2018Article
- Emerging Concepts in Brain Glucose Metabolic Functions: From Glucose Sensing to How the Sweet Taste of Glucose Regulates Its Own Metabolism in Astrocytes and Neurons.Neuromolecular medicine · 2018Review
- Review
- Lactate in the brain: from metabolic end-product to signalling molecule.Nature reviews. Neuroscience · 2018Review
- Neuronal Stimulation Triggers Neuronal Glycolysis and Not Lactate Uptake.Cell metabolism · 2017Article
- Lactate Shuttles in Neuroenergetics-Homeostasis, Allostasis and Beyond.Frontiers in neuroscience · 2017Review
- Effects of Systemic Metabolic Fuels on Glucose and Lactate Levels in the Brain Extracellular Compartment of the Mouse.Frontiers in neuroscience · 2017Article
- Bioenergetics and redox adaptations of astrocytes to neuronal activity.Journal of neurochemistry · 2016Review
- L-Lactate protects neurons against excitotoxicity: implication of an ATP-mediated signaling cascade.Scientific reports · 2016Article
- Fluctuations in Cytosolic Calcium Regulate the Neuronal Malate-Aspartate NADH Shuttle: Implications for Neuronal Energy Metabolism.Neurochemical research · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although it is known that brain activity is fueled by glucose, the identity of the cell type that preferentially metabolizes the sugar remains elusive. To address this question, glucose uptake was studied simultaneously in cultured hippocampal neurons and neighboring astrocytes using a real-time assay based on confocal epifluorescence microscopy and fluorescent glucose analogs. Glutamate, although stimulating glucose transport in astrocytes, strongly inhibited glucose transport in neurons, producing in few seconds a 12-fold increase in the ratio of astrocytic-to-neuronal uptake rate. Neuronal transport inhibition was reversible on removal of the neurotransmitter and displayed an IC50 of 5 microm, suggesting its occurrence at physiological glutamate concentrations. The phenomenon was abolished by CNQX and mimicked by AMPA, demonstrating a role for the cognate subset of ionotropic glutamate receptors. Transport inhibition required extracellular sodium and calcium and was mimicked by veratridine but not by membrane depolarization with high K+ or by calcium overloading with ionomycin. Therefore, glutamate inhibits glucose transport via AMPA receptor-mediated sodium entry, whereas calcium entry plays a permissive role. This phenomenon suggests that glutamate redistributes glucose toward astrocytes and away from neurons and represents a novel molecular mechanism that may be important for functional imaging of the brain using positron emission tomography.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.