ArticleMolecular biology of the cell1992
Influence of gamma subunit prenylation on association of guanine nucleotide-binding regulatory proteins with membranes.
Article in Molecular biology of the cell, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 154 citations in OpenAlex.
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- Negative Feedback Phosphorylation of Gγ Subunit Ste18 and the Ste5 Scaffold Synergistically Regulates MAPK Activation in Yeast.Cell reports · 2018Article
- Mutation of 3-hydroxy-3-methylglutaryl CoA synthase I reveals requirements for isoprenoid and cholesterol synthesis in oligodendrocyte migration arrest, axon wrapping, and myelin gene expression.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- The Gβ3 splice variant associated with the C825T gene polymorphism is an unstable and functionally inactive protein.Cellular signalling · 2012Article
- Nucleotide exchange factors: Kinetic analyses and the rationale for studying kinetics of GEFs.Cellular logistics · 2012Article
- Effects of 4-week administration of simvastatin in different doses on heart rate and blood pressure after metoprolol injection in normocholesterolaemic and normotensive rats.Archives of medical science : AMS · 2012Article
- Central and C-terminal domains of heterotrimeric G protein gamma subunits differentially influence the signaling necessary for primordial germ cell migration.Cellular signalling · 2011Article
- Adenosine A2A receptor signaling and golf assembly show a specific requirement for the gamma7 subtype in the striatum.The Journal of biological chemistry · 2010Article
- Prenylation-deficient G protein gamma subunits disrupt GPCR signaling in the zebrafish.Cellular signalling · 2010Article
- The phosducin-like protein PhLP1 is essential for G{beta}{gamma} dimer formation in Dictyostelium discoideum.Molecular and cellular biology · 2005Article
- Gi protein activation in intact cells involves subunit rearrangement rather than dissociation.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Disruption of hepatitis C virus RNA replication through inhibition of host protein geranylgeranylation.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins.The EMBO journal · 2003Article
- Identification of a region in G protein gamma subunits conserved across species but hypervariable among subunit isoforms.Protein science : a publication of the Protein Society · 2001Article
- Ggamma in dictyostelium: its role in localization of gbetagamma to the membrane is required for chemotaxis in shallow gradients.Molecular biology of the cell · 2001Article
- Independent and synergistic interaction of retinal G-protein subunits with bovine rhodopsin measured by surface plasmon resonance.The Biochemical journal · 2001Article
- Localization of a peripheral membrane protein: Gbetagamma targets Galpha(Z).Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Two approaches were taken to address the possible role of gamma-subunit prenylation in dictating the cellular distribution of guanine nucleotide-binding regulatory proteins. Prenylation of gamma subunits was prevented by site-directed mutagenesis or by inhibiting the synthesis of mevalonate, the precursor of cellular isoprenoids. When beta or gamma subunits were transiently expressed in COS-M6 simian kidney cells (COS) cells, the proteins were found in the membrane fraction by immunoblotting. Immunofluorescence experiments indicated that the proteins were distributed to intracellular structures in addition to plasma membranes. Replacement of Cys68 of gamma with Ser prevented prenylation of the mutant protein and association of the protein with the membrane fraction of COS cells. Immunoblotting results demonstrated that some of the beta subunits were found in the cytoplasm when coexpressed with the nonprenylated mutant gamma subunit. When Neuro 2A cells were treated with compactin to inhibit protein prenylation, a fraction of endogenous beta and gamma was distributed in the cytoplasm. It is concluded that prenylation facilitates association of gamma subunits with membranes, that the cellular location of gamma influences the distribution of beta, and that prenylation is not an absolute requirement for interaction of beta and gamma.
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