ArticleNucleic acids research2004
Guanosine tetra- and pentaphosphate synthase activity in chloroplasts of a higher plant: association with 70S ribosomes and inhibition by tetracycline.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 77 citations in OpenAlex.
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Critical stages in pea photosynthesis impaired by tetracycline as an environmental contaminant.Journal of plant research · 2024Article
- Article
- The Importance of Microorganisms for Sustainable Agriculture-A Review.Metabolites · 2022Review
- Toxicity of chlortetracycline and oxytetracycline on Vallisneria natans (Lour.) Hare.Environmental science and pollution research international · 2021Article
- Review
- Antibiotics impact plant traits, even at small concentrations.AoB PLANTS · 2017Article
- Antibiotic use and abuse: a threat to mitochondria and chloroplasts with impact on research, health, and environment.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- Effects of tetracycline on developmental toxicity and molecular responses in zebrafish (Danio rerio) embryos.Ecotoxicology (London, England) · 2015Article
- Diversity in guanosine 3',5'-bisdiphosphate (ppGpp) sensitivity among guanylate kinases of bacteria and plants.The Journal of biological chemistry · 2014Article
- Biosolid-borne tetracyclines and sulfonamides in plants.Environmental science and pollution research international · 2013Review
- Review
- ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro.Plant molecular biology · 2012Article
- Function of plastid sigma factors in higher plants: regulation of gene expression or just preservation of constitutive transcription?Plant molecular biology · 2011Article
- Multiple antibiotic resistance in Arabidopsis is conferred by mutations in a chloroplast-localized transport protein.Plant physiology · 2009Article
- Plant sigma factors and their role in plastid transcription.Plant cell reports · 2007Review
- Physiological analysis of the stringent response elicited in an extreme thermophilic bacterium, Thermus thermophilus.Journal of bacteriology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplasts possess bacterial-type systems for transcription and translation. On the basis of the identification of a Chlamydomonas reinhardtii gene encoding a RelA-SpoT homolog (RSH) that catalyzes the synthesis of guanosine tetra- or pentaphosphate [(p)ppGpp], we have previously suggested the operation of stringent control in the chloroplast genetic system. Although RSH genes have also been identified in several higher plants, the activities of the encoded enzymes and their mode of action in chloroplasts have remained uncharacterized. We have now characterized the intrinsic (p)ppGpp synthase activity of chloroplast extracts prepared from pea (Pisum sativum). Fractionation by ultracentrifugation suggested that the (p)ppGpp synthase activity of a translationally active chloroplast stromal extract was associated with 70S ribosomes. Furthermore, this enzymatic activity was inhibited by tetracycline, as was the peptide elongation activity of the extract. Structural comparisons between rRNA molecules of Escherichia coli and pea chloroplasts revealed the conservation of putative tetracycline-binding sites. These observations demonstrate the presence of a ribosome-associated (p)ppGpp synthase activity in the chloroplasts of a higher plant, further implicating (p)ppGpp in a genetic system of chloroplasts similar to that operative in bacteria.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.