ArticleThe Journal of molecular diagnostics : JMD2004
Enhanced retrieval of DNA from human fecal samples results in improved performance of colorectal cancer screening test.
Article in The Journal of molecular diagnostics : JMD, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Early detection of colorectal cancer based on presence of methylated syndecan-2 (SDC2) in stool DNA.Clinical epigenetics · 2019Trial
- Stool DNA and occult blood testing for screen detection of colorectal neoplasia.Annals of internal medicine · 2008Trial
- Beyond colonoscopy, faecal DNA mutation screening provides a potential and viable path to early colorectal cancer detection.Scientific reports · 2025Article
- Comparison of Fecal DNA Extraction Kits for the Giant Panda (Ecology and evolution · 2025Article
- High-throughput, pan-leukocyte biomarkers for the detection of inflammation in human breastmilk and stool.Frontiers in epigenetics and epigenomics · 2025Article
- Detection of Human Y Chromosome and theGastro hep advances · 2025Article
- Circulating Nucleic Acid-Based Biomarkers of Type 2 Diabetes.International journal of molecular sciences · 2021Review
- Selective capture of plasma cell-free tumor DNA on magnetic beads: a sensitive and versatile tool for liquid biopsy.Cellular oncology (Dordrecht, Netherlands) · 2020Article
- Methylation-based enrichment facilitates low-cost, noninvasive genomic scale sequencing of populations from feces.Scientific reports · 2018Article
- Feasibility of quantifyingClinical epigenetics · 2017Article
- Tests detecting biomarkers for screening of colorectal cancer: What is on the horizon?GMS health technology assessment · 2015Article
- Circulating and stool nucleic acid analysis for colorectal cancer diagnosis.World journal of gastroenterology · 2014Review
- Analytical sensitivity and stability of DNA methylation testing in stool samples for colorectal cancer detection.Cellular oncology (Dordrecht, Netherlands) · 2012Article
- Dual probe DNA capture for sensitive real-time PCR detection of Cryptosporidium and Giardia.Molecular and cellular probes · 2012Article
- Protein biomarkers in exfoliated cells collected from the human rectal mucosa: implications for colorectal disease detection and monitoring.International journal of colorectal disease · 2011Article
- Stool DNA testing to screen for colorectal cancer in the Medicare population: a cost-effectiveness analysis.Annals of internal medicine · 2010Article
- Can DNA sampling from the rectal mucosa be a novel tool for the detection of colorectal cancer?International journal of colorectal disease · 2010Article
- Genetic and epigenetic marker-based DNA test of stool is a promising approach for colorectal cancer screening.Yonsei medical journal · 2009Review
- Implications of new colorectal cancer screening technologies for primary care practice.Medical care · 2008Review
- Analysis of mutations in DNA isolated from plasma and stool of colorectal cancer patients.Gastroenterology · 2008Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Colorectal cancer accounts for more than 10% of all cancer deaths but is curable, if detected early. We reported previously on a stool-based screening test in which DNA from stool samples is subjected to genome analysis; sensitivity of the test has been limited in part by inefficiency of retrieving DNA from stool. Our aim was to test the impact of a new purification method that would increase the yield of human DNA from stool. DNA from 86 cancer and 100 non-cancer subjects (diagnosed by colonoscopy) were purified from stool with a new method for DNA recovery based on sequence-specific capture with acrylamide gel immobilized capture probes as well as with a previously developed magnetic bead-capture procedure. The new purification method gives an average 5.4-fold increase in the quantity of human DNA that can routinely be retrieved from fecal samples. The increased recovery of DNA corresponds with an increase in assay sensitivity from 53% (CI: 42 to 64%) to 70% (CI: 59 to 79%); P = 0.0005 (by McNemar's test), with no change in specificity. The newly developed sample preparation method mitigates a major problem in detecting rare cancer-associated genetic changes in heterogeneous clinical samples such as stool.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.