Evidence map›Paper›PMID 15507648›Full record

ArticleJournal of virology2004

Epstein-Barr virus infection in ex vivo tonsil epithelial cell cultures of asymptomatic carriers.

Dirk M Pegtel, Jaap Middeldorp, David A Thorley-Lawson

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed
4.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 128 citations in OpenAlex.

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2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Dirk M PegtelDepartment of Pathology, Tufts University School of Medicine, Jaharis Bldg., 150 Harrison Avenue, Boston, MA 02111, USA.
Jaap Middeldorp
David A Thorley-Lawson
Tufts University · USVrije Universiteit Amsterdam · NL

Funding

HOST IMMUNITY TO EBV INFECTION IN VITRO AND IN VIVOR01AI018757 · NIAID · TUFTS UNIVERSITY BOSTON · PI POLTORAK, ALEXANDER · 1985 to 2017
$6.7M
EPSTEIN BARR VIRUS LATENCYR01CA065883 · NCI · TUFTS UNIVERSITY BOSTON · PI THORLEY-LAWSON, DAVID A. · 1995 to 2014
$4.3M
NCI NIH HHS CA 65883NCI NIH HHS R01 CA065883NIAID NIH HHS AI 18757NIAID NIH HHS R01 AI018757
6 · The paper itself

Abstract

Epstein-Barr virus (EBV) is found frequently in certain epithelial pathologies, such as nasopharyngeal carcinoma and oral hairy leukoplakia, indicating that the virus can infect epithelial cells in vivo. Recent studies of cell lines imply that epithelial cells may also play a role in persistent EBV infection in vivo. In this report, we show the establishment and characterization of an ex vivo culture model of tonsil epithelial cells, a likely site for EBV infection in vivo. Primary epithelial-cell cultures, generated from tonsil explants, contained a heterogeneous mixture of cells with an ongoing process of differentiation. Keratin expression profiles were consistent with the presence of cells from both surface and crypt epithelia. A small subset of cells could be latently infected by coculture with EBV-releasing cell lines, but not with cell-free virus. We also detected viral-DNA, -mRNA, and -protein expression in cultures from EBV-positive tonsil donors prior to in vitro infection. We conclude that these cells were either already infected at the time of explantation or soon after through cell-to-cell contact with B cells replicating EBV in the explant. Taken together, these findings suggest that the tonsil epithelium of asymptomatic virus carriers is able to sustain EBV infection in vivo. This provides an explanation for the presence of EBV in naso- and oropharyngeal pathologies and is consistent with epithelial cells playing a role in the egress of EBV during persistent infection.

Indexed as

AdolescentCarrier StateCells, CulturedChildEpithelial CellsHerpesvirus 4, HumanHumansPalatine TonsilViral Matrix ProteinsEBV-associated membrane antigen, Epstein-Barr virusViral Matrix Proteins

Identifiers

PMID15507648
PMCPMC525079
OpenAlexW2160746136

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.