ArticleJournal of virology2004
Tracking fluorescence-labeled rabies virus: enhanced green fluorescent protein-tagged phosphoprotein P supports virus gene expression and formation of infectious particles.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 82 citations in OpenAlex.
- Rabies lyssavirus phosphoprotein possesses RNA duplex-unwinding activities.Molecular therapy. Nucleic acids · 2026Article
- "Self-inactivating" rabies viruses are susceptible to loss of their intended attenuating modification.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Revealing the Cell Entry Dynamic Mechanism of Single Rabies Virus Particle.Chemical research in Chinese universities · 2022Article
- Chemogenetic ON and OFF switches for RNA virus replication.Nature communications · 2021Article
- Establishment of a longitudinal pre-clinical model of lyssavirus infection.Journal of virological methods · 2020Article
- A materials-science perspective on tackling COVID-19.Nature reviews. Materials · 2020Review
- Specificity of Plant Rhabdovirus Cell-to-Cell Movement.Journal of virology · 2019Article
- Novel mass spectrometry based detection and identification of variants of rabies virus nucleoprotein in infected brain tissues.PLoS neglected tropical diseases · 2018Article
- Retrograde axonal transport of rabies virus is unaffected by interferon treatment but blocked by emetine locally in axons.PLoS pathogens · 2018Article
- Single-molecule fluorescence imaging: Generating insights into molecular interactions in virology.Journal of biosciences · 2018Review
- Imaging, Tracking and Computational Analyses of Virus Entry and Egress with the Cytoskeleton.Viruses · 2018Review
- Everything You Always Wanted to Know About Rabies Virus (But Were Afraid to Ask).Annual review of virology · 2015Review
- A Dynein Light Chain 1 Binding Motif in Rabies Virus Polymerase L Protein Plays a Role in Microtubule Reorganization and Viral Primary Transcription.Journal of virology · 2015Article
- Axonal spread of neuroinvasive viral infections.Trends in microbiology · 2015Review
- Interactome analysis of the human respiratory syncytial virus RNA polymerase complex identifies protein chaperones as important cofactors that promote L-protein stability and RNA synthesis.Journal of virology · 2015Article
- Anterograde glycoprotein-dependent transport of newly generated rabies virus in dorsal root ganglion neurons.Journal of virology · 2014Article
- Current status of rabies and prospects for elimination.Lancet (London, England) · 2014Review
- Rabies Virus Hijacks and accelerates the p75NTR retrograde axonal transport machinery.PLoS pathogens · 2014Article
- Generation of recombinant rabies Virus CVS-11 expressing eGFP applied to the rapid virus neutralization test.Viruses · 2014Article
- Development and application of reporter-expressing mononegaviruses: current challenges and perspectives.Antiviral research · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhabdoviruses such as rabies virus (RV) encode only five multifunctional proteins accomplishing viral gene expression and virus formation. The viral phosphoprotein, P, is a structural component of the viral ribonucleoprotein (RNP) complex and an essential cofactor for the viral RNA-dependent RNA polymerase. We show here that RV P fused to enhanced green fluorescent protein (eGFP) can substitute for P throughout the viral life cycle, allowing fluorescence labeling and tracking of RV RNPs under live cell conditions. To first assess the functions of P fusion constructs, a recombinant RV lacking the P gene, SAD DeltaP, was complemented in cell lines constitutively expressing eGFP-P or P-eGFP fusion proteins. P-eGFP supported the rapid accumulation of viral mRNAs but led to low infectious-virus titers, suggesting impairment of virus formation. In contrast, complementation with eGFP-P resulted in slower accumulation of mRNAs but similar infectious titers, suggesting interference with polymerase activity rather than with virus formation. Fluorescence microscopy allowed the detection of eGFP-P-labeled extracellular virus particles and tracking of cell binding and temperature-dependent internalization into intracellular vesicles. Recombinant RVs expressing eGFP-P or an eGFP-P mutant lacking the binding site for dynein light chain 1 (DLC1) instead of P were used to track interaction with cellular proteins. In cells expressing a DsRed-labeled DLC1, colocalization of DLC1 with eGFP-P but not with the mutant P was observed. Fluorescent labeling of RV RNPs will allow further dissection of virus entry, replication, and egress under live-cell conditions as well as cell interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.