ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2004
Spatial distribution and initial changes of SSEA-1 and other cell adhesion-related molecules on mouse embryonic stem cells before and during differentiation.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 94 citations in OpenAlex.
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- Endometrial stem/progenitor cells: Properties, origins, and functions.Genes & diseases · 2023Review
- Naïve pluripotent-like characteristics of non-tumorigenic Muse cells isolated from human amniotic membrane.Scientific reports · 2022Article
- Neonatal lung-derived SSEA-1iScience · 2022Article
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- Asymptomatic neurotoxicity of amyloid β-peptides (Aβ1-42 and Aβ25-35) on mouse embryonic stem cell-derived neural cells.Bosnian journal of basic medical sciences · 2021Article
- Primordial germ cells can be differentiated by retinoic acid and progesterone induction from embryonic stem cells.Journal of biosciences · 2021Article
- Inhibition of JAK-STAT Signaling Pathway Alleviates Age-Related Phenotypes in Tendon Stem/Progenitor Cells.Frontiers in cell and developmental biology · 2021Article
- Increased Expression of Cell Surface SSEA-1 is Closely Associated with Naïve-Like Conversion from Human Deciduous Teeth Dental Pulp Cells-Derived iPS Cells.International journal of molecular sciences · 2019Article
- Media composition modulates human embryonic stem cell morphology and may influence preferential lineage differentiation potential.PloS one · 2019Article
- Correlating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAScientific reports · 2017Article
- Mouse embryonic dorsal root ganglia contain pluripotent stem cells that show features similar to embryonic stem cells and induced pluripotent stem cells.Biology open · 2017Article
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- Protein profile of basal prostate epithelial progenitor cells--stage-specific embryonal antigen 4 expressing cells have enhanced regenerative potential in vivo.Journal of cellular and molecular medicine · 2016Article
- Phenotype and Stability of Neural Differentiation of Androgenetic Murine ES Cell-Derived Neural Progenitor Cells.Cell medicine · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We examined the distribution of cell adhesion-related molecules (CAMs) among mouse embryonic stem (ES) cells and the spatial distribution on cell surfaces before and during differentiation. The cell-cell heterogeneity of SSEA-1, PECAM-1, and ICAM-1 among the undifferentiated cells in the ES cell colonies was evident by immunohistochemistry and immuno-SEM, supporting the flow cytometry findings. In contrast, most undifferentiated ES cells strongly expressed CD9. SSEA-1 was located preferentially on the edge of low protuberances and microvilli and formed clusters or linear arrays of 3-20 particles. PECAM-1 and ICAM-1 were randomly localized on the free cell surfaces, whereas CD9 was preferentially localized on the microvilli or protuberances, especially in the cell periphery. Both the SSEA-1(+) fraction and the SSEA-1(-) fraction of magnetic cell sorting (MACS) formed undifferentiated colonies after plating. Flow cytometry showed that these populations reverted separately again to a culture with a mixed phenotype. Differentiation induced by retinoic acid downregulated the expression of all CAMs. Immuno-SEM showed decreases of SSEA-1 in the differentiated ES cells, although some clustering still remained. Our findings help to elucidate the significance of these molecules in ES cell maintenance and differentiation and suggest that cell surface antigens may be useful for defining the phenotype of undifferentiated and differentiated ES cells.
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