ArticleInvestigative ophthalmology & visual science2004
Hypoxia increases corneal cell expression of CFTR leading to increased Pseudomonas aeruginosa binding, internalization, and initiation of inflammation.
Article in Investigative ophthalmology & visual science, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Contact Lens-Associated Ocular Surface and Corneal Disorders.Methods and protocols · 2026Review
- Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis.Nature communications · 2025Article
- Evidence for intracellularJournal of bacteriology · 2024Review
- Hypoxia adaptation in the cornea: Current animal models and underlying mechanisms.Animal models and experimental medicine · 2021Review
- Foundational concepts in the biology of bacterial keratitis.Experimental eye research · 2021Review
- Transcriptome Analysis of the Gene Expression Profiles Associated with Fungal Keratitis in Mice Based on RNA-Seq.Investigative ophthalmology & visual science · 2020Article
- The Role of Hypoxia in Corneal Extracellular Matrix Deposition and Cell Motility.Anatomical record (Hoboken, N.J. : 2007) · 2020Article
- The Role ofPathogens (Basel, Switzerland) · 2019Review
- Repair Process Impairment byFrontiers in cellular and infection microbiology · 2019Review
- Cystic fibrosis transmembrane conductance regulator-emerging regulator of cancer.Cellular and molecular life sciences : CMLS · 2018Review
- Ion channels of the lung and their role in disease pathogenesis.American journal of physiology. Lung cellular and molecular physiology · 2017Review
- Second harmonic generation imaging of corneal stroma after infection by Pseudomonas aeruginosa.Scientific reports · 2017Article
- Pseudomonas aeruginosa infectious keratitis in a high oxygen transmissible rigid contact lens rabbit model.Investigative ophthalmology & visual science · 2014Article
- Article
- The effects of silicone hydrogel lens wear on the corneal epithelium and risk for microbial keratitis.Eye & contact lens · 2013Review
- Chloride channels and transporters in human corneal epithelium.Experimental eye research · 2010Article
- Pathogenesis of contact lens-associated microbial keratitis.Optometry and vision science : official publication of the American Academy of Optometry · 2010Review
- Hypoxia inducible factor-1 (HIF-1)-mediated repression of cystic fibrosis transmembrane conductance regulator (CFTR) in the intestinal epithelium.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Role of oxygen availability in CFTR expression and function.American journal of respiratory cell and molecular biology · 2008Article
- Disruption of CFTR-dependent lipid rafts reduces bacterial levels and corneal disease in a murine model of Pseudomonas aeruginosa keratitis.Investigative ophthalmology & visual science · 2008Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo investigate the effect of hypoxia-induced molecular responses of corneal epithelial cells on the surface of rabbit and human corneas and corneal cells in culture on interactions with Pseudomonas aeruginosa that may underlie increased susceptibility to keratitis.
methodsOrgan cultures of rabbit and human corneal tissue, primary rabbit and human corneal cells, and transformed human corneal cells from a patient with cystic fibrosis and the same cell line corrected for expression of wild-type cystic fibrosis transmembrane conductance regulator (CFTR), the cellular receptor for P. aeruginosa, were exposed to hypoxic conditions for 24 to 72 hours. Changes in binding and internalization of P. aeruginosa were measured using cellular association and gentamicin-exclusion assays, and expression of CFTR and activation of NF-kappaB in response to hypoxia were determined by confocal laser microscopy and quantitative measurements of NF-kappaB activation.
resultsHypoxia induced in a time- and oxygen-concentration-dependent manner increased association and internalization of clinical isolates of P. aeruginosa in all cells tested. Hypoxia increased CFTR expression and NF-kappaB nuclear translocation in rabbit and human cells with wild-type CFTR. Corneal cells lacking CFTR had reduced NF-kappaB activation in response to hypoxia. Hypoxia did not affect the increase in corneal cell CFTR levels or NF-kappaB activation after P. aeruginosa infection.
conclusionsHypoxic conditions on the cornea exacerbate the binding and internalization of P. aeruginosa due to increased levels of CFTR expression and also induce basal NF-kappaB activation. Both of these responses probably exacerbate the effects of P. aeruginosa infection by allowing lower infectious doses of bacteria to induce disease and promote destructive inflammatory responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.