ArticleThe Journal of cell biology2004
UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 131 citations in OpenAlex.
- The MO25 protein Pmo25 functions in contractile ring stability and Sid2 localization during cytokinesis.iScience · 2026Article
- Actin arginylation alters myosin engagement and F-actin patterning despite structural conservation.The Journal of cell biology · 2026Article
- The Ync13-Rga7-Rng10 complex selectively coordinates secretory vesicle trafficking and secondary septum formation during cytokinesis.PLoS biology · 2025Article
- Roles of the MO25 protein Pmo25 in contractile-ring stability and localization of the NDR kinase Sid2 during cytokinesis.bioRxiv : the preprint server for biology · 2025Article
- Spatial control of secretory vesicle targeting by the Ync13-Rga7-Rng10 complex during cytokinesis.bioRxiv : the preprint server for biology · 2025Article
- The Mechanosensitive Pkd2 Channel Modulates the Recruitment of Myosin II and Actin to the Cytokinetic Contractile Ring.Journal of fungi (Basel, Switzerland) · 2024Article
- SPR spectroscopic analysis of myosin binding to wild type and mutant UNC45B.microPublication biology · 2024Article
- Actin turnover protects the cytokinetic contractile ring from structural instability.Journal of cell science · 2023Article
- UCS Chaperone Folding of the Myosin Head: A Function That Evolved before Animals and Fungi Diverged from a Common Ancestor More than a Billion Years Ago.Biomolecules · 2022Review
- Laser ablation reveals the impact of Cdc15p on the stiffness of the contractile ring.Molecular biology of the cell · 2022Article
- Escape from mitotic catastrophe by actin-dependent nuclear displacement in fission yeast.iScience · 2021Article
- Effects of Mutations in the Phenamacril-Binding Site ofACS omega · 2020Article
- Mechanism of Long-Range Chromosome Motion Triggered by Gene Activation.Developmental cell · 2020Article
- Comparative Analysis of the Roles of Non-muscle Myosin-IIs in Cytokinesis in Budding Yeast, Fission Yeast, and Mammalian Cells.Frontiers in cell and developmental biology · 2020Review
- The Functionally Important N-Terminal Half of Fission Yeast Mid1p Anillin Is Intrinsically Disordered and Undergoes Phase Separation.Biochemistry · 2019Article
- Molecular Mechanism of Cytokinesis.Annual review of biochemistry · 2019Review
- Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed Conditions.iScience · 2019Article
- Mechanisms of contractile ring tension production and constriction.Biophysical reviews · 2018Review
- UCS protein function is partially restored in the Saccharomyces cerevisiae she4 mutant with expression of the human UNC45-GC, but not UNC45-SM.Cell stress & chaperones · 2018Article
- Coarse-grained simulations of actomyosin rings point to a nodeless model involving both unipolar and bipolar myosins.Molecular biology of the cell · 2018Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We purified native Myo2p/Cdc4p/Rlc1p (Myo2), the myosin-II motor required for cytokinesis by Schizosaccharomyces pombe. The Myo2p heavy chain associates with two light chains, Cdc4p and Rlc1p. Although crude Myo2 supported gliding motility of actin filaments in vitro, purified Myo2 lacked this activity in spite of retaining full Ca-ATPase activity and partial actin-activated Mg-ATPase activity. Unc45-/Cro1p-/She4p-related (UCS) protein Rng3p restored the full motility and actin-activated Mg-ATPase activity of purified Myo2. The COOH-terminal UCS domain of Rng3p alone restored motility to pure Myo2. Thus, Rng3p contributes directly to the motility activity of native Myo2. Consistent with a role in Myo2 activation, Rng3p colocalizes with Myo2p in the cytokinetic contractile ring. The absence of Rlc1p or mutations in the Myo2p head or Rng3p compromise the in vitro motility of Myo2 and explain the defects in cytokinesis associated with some of these mutations. In contrast, Myo2 with certain temperature-sensitive forms of Cdc4p has normal motility, so these mutations compromise other functions of Cdc4p required for cytokinesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.