ArticleAntimicrobial agents and chemotherapy2004
Rapid direct detection of multiple rifampin and isoniazid resistance mutations in Mycobacterium tuberculosis in respiratory samples by real-time PCR.
Article in Antimicrobial agents and chemotherapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 62 citations in OpenAlex.
- Laboratory tools for the direct detection of bacterial respiratory infections and antimicrobial resistance: a scoping review.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Detection ofPathogens (Basel, Switzerland) · 2023Article
- Utility of Real-Time Quantitative Polymerase Chain Reaction in DetectingBioMed research international · 2017Article
- Rapid Detection of Rifampicin- and Isoniazid-ResistantJundishapur journal of microbiology · 2016Article
- Rapid Detection of Rifampicin- and Isoniazid-Resistant Mycobacterium tuberculosis using TaqMan Allelic Discrimination.Osong public health and research perspectives · 2016Article
- Multi-Fluorescence Real-Time PCR Assay for Detection of RIF and INH Resistance of M. tuberculosis.Frontiers in microbiology · 2016Article
- Rapid detection of MDR-Mycobacterium tuberculosis using modified PCR-SSCP from clinical Specimens.Asian Pacific journal of tropical biomedicine · 2014Article
- Development of a novel PCR restriction analysis of the hsp65 gene as a rapid method to screen for the Mycobacterium tuberculosis complex and nontuberculous mycobacteria in high-burden countries.Journal of clinical microbiology · 2013Article
- Fast test for assessing the susceptibility of Mycobacterium tuberculosis to isoniazid and rifampin by real-time PCR.Memorias do Instituto Oswaldo Cruz · 2012Article
- Rapid, high-throughput detection of rifampin resistance and heteroresistance in Mycobacterium tuberculosis by use of sloppy molecular beacon melting temperature coding.Journal of clinical microbiology · 2012Article
- Rapid detection of rpoB mutations in rifampin resistant M. tuberculosis from sputum samples by denaturing gradient gel electrophoresis.International journal of medical sciences · 2012Article
- Multiplex real-time PCR melting curve assay to detect drug-resistant mutations of Mycobacterium tuberculosis.Journal of clinical microbiology · 2011Article
- Evaluation of Cobas TaqMan MTB PCR for detection of Mycobacterium tuberculosis.Journal of clinical microbiology · 2011Article
- High-level rifampin resistance correlates with multiple mutations in the rpoB gene of pulmonary tuberculosis isolates from the Afghanistan border of Iran.Journal of clinical microbiology · 2009Article
- Molecular diagnostics in tuberculosis: basis and implications for therapy.Molecular diagnosis & therapy · 2009Review
- Novel wide-range quantitative nested real-time PCR assay for Mycobacterium tuberculosis DNA: clinical application for diagnosis of tuberculous meningitis.Journal of clinical microbiology · 2008Article
- Novel wide-range quantitative nested real-time PCR assay for Mycobacterium tuberculosis DNA: development and methodology.Journal of clinical microbiology · 2008Article
- Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes.Current genomics · 2007Article
- Rapid genotypic detection of rifampin- and isoniazid-resistant Mycobacterium tuberculosis directly in clinical specimens.Journal of clinical microbiology · 2006Article
- Novel technique of quantitative nested real-time PCR assay for Mycobacterium tuberculosis DNA.Journal of clinical microbiology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid detection of resistance in Mycobacterium tuberculosis can optimize the efficacy of antituberculous therapy and control the transmission of resistant M. tuberculosis strains. Real-time PCR has minimized the time required to obtain the susceptibility pattern of M. tuberculosis strains, but little effort has been made to adapt this rapid technique to the direct detection of resistance from clinical samples. In this study, we adapted and evaluated a real-time PCR design for direct detection of resistance mutations in clinical respiratory samples. The real-time PCR was evaluated with (i) 11 clinical respiratory samples harboring bacilli resistant to isoniazid (INH) and/or rifampin (RIF), (ii) 10 culture-negative sputa spiked with a set of strains encoding 14 different resistance mutations in 10 independent codons, and (iii) 16 sputa harboring susceptible strains. The results obtained with this real-time PCR design completely agreed with DNA sequencing data. In all sputa harboring resistant M. tuberculosis strains, the mutation encoding resistance was successfully detected. No mutation was detected in any of the susceptible sputa. The test was applied only to smear-positive specimens and succeeded in detecting a bacterial load equivalent to 10(3) CFU/ml in sputum samples (10 acid-fast bacilli/line). The analytical specificity of this method was proved with a set of 14 different non-M. tuberculosis bacteria. This real-time PCR design is an adequate method for the specific and rapid detection of RIF and INH resistance in smear-positive clinical respiratory samples.
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