ArticlePlant cell reports2005
Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato.
Article in Plant cell reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 129 citations in OpenAlex.
- RNAi: the next wave of green agriculture, challenges, and bridging the translational gap.Plant cell reports · 2026Review
- Quorum quenching, lactic acid bacteria and microencapsulation as emerging and promising strategies to mitigate tomato soft rot.Frontiers in plant science · 2026Review
- Role of biotechnology for shelf-life extension and quality improvement of perishable fruits and vegetables: a comprehensive review.Food science and biotechnology · 2025Review
- Chemical induction of DNA demethylation by 5-Azacytidine enhances tomato fruit defense against gray mold through dicer-like proteinHorticulture research · 2024Article
- Antisense RNA (asRNA) technology: the concept and applications in crop improvement and sustainable agriculture.Molecular biology reports · 2023Review
- Quality trait improvement in horticultural crops: OMICS and modern biotechnological approaches.Molecular biology reports · 2023Review
- RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture.Plants (Basel, Switzerland) · 2021Review
- Discovery and Characterization of a Novel TomatoGenes · 2021Article
- Can gene editing reduce postharvest waste and loss of fruit, vegetables, and ornamentals?Horticulture research · 2021Review
- Application of Exogenous dsRNAs-induced RNAi in Agriculture: Challenges and Triumphs.Frontiers in plant science · 2020Review
- 1-Aminocyclopropane-1-Carboxylic Acid Oxidase (ACO): The Enzyme That Makes the Plant Hormone Ethylene.Frontiers in plant science · 2019Review
- Article
- RNA Silencing in Plants: Mechanisms, Technologies and Applications in Horticultural Crops.Current genomics · 2016Article
- Global Transcriptomic Analysis of Targeted Silencing of Two Paralogous ACC Oxidase Genes in Banana.International journal of molecular sciences · 2016Article
- Overexpression of plum auxin receptor PslTIR1 in tomato alters plant growth, fruit development and fruit shelf-life characteristics.BMC plant biology · 2016Article
- Over-expression of mouse ornithine decarboxylase gene under the control of fruit-specific promoter enhances fruit quality in tomato.Plant molecular biology · 2015Article
- Small RNAs in plants: recent development and application for crop improvement.Frontiers in plant science · 2015Review
- Review
- Temporal and spatial control of gene expression in horticultural crops.Horticulture research · 2014Review
- RNA Interference (RNAi) Induced Gene Silencing: A Promising Approach of Hi-Tech Plant Breeding.International journal of biological sciences · 2014Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) is a potent trigger for specific gene silencing of expression in a number of organisms and is an efficient way of shutting down gene expression. 1-Aminocyclopropane-1-carboxylate (ACC) oxidase catalyzes the oxidation of ACC to ethylene, a plant growth regulator that plays an important role in the tomato ripening process. In this research, to produce double-stranded (ds)RNA of tomato ACC oxidase, we linked the sense and antisense configurations of DNA fragments with 1,002-bp or 7-nt artificially synthesized fragments, respectively, and then placed these under the control of a modified cauliflower mosaic virus 35S promoter. The dsRNA expression unit was successfully introduced into tomato cultivar Hezuo 906 by Agrobacterium tumefaciens-mediated transformation. Molecular analysis of 183 transgenic plants revealed that the dsRNA unit was integrated into the tomato genome. With respect to the construct with the 1,002-bp linker, the severity of phenotypes indicated that 72.3% of the transformed plants had non-RNA interference, about 18.1% had semi-RNA interference, and only 9.6% had full-RNA interference. However when the construct with the 7-nt linker was used for transformation, the results were 13.0%, 18.0%, and 69.0%, respectively, indicating that the short linker was more efficient in RNAi of transgenic tomato plants. When we applied this fast way of shutting down the ACC oxidase gene, transgenic tomato plants were produced that had fruit which released traces of ethylene and had a prolonged shelf life of more than 120 days. The RNA and protein analyses indicated that there was non-RNA interference, semi-RNA interference and full-RNA interference of ACC oxidase in the transgenic tomato plants.
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