ArticlePlant cell reports2005
A rapid and efficient transformation protocol for the grass Brachypodium distachyon.
Article in Plant cell reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 86 citations in OpenAlex.
- Fast-track transformation and genome editing in Brachypodium distachyon.Plant methods · 2023Article
- The auxin response factor TaARF15-A1 negatively regulates senescence in common wheat (Triticum aestivum L.).Plant physiology · 2023Article
- One-Week Scutellar Somatic Embryogenesis in the MonocotPlants (Basel, Switzerland) · 2022Article
- Brachypodium distachyon UNICULME4 and LAXATUM-A are redundantly required for development.Plant physiology · 2022Article
- Artificial chromosome technology and its potential application in plants.Frontiers in plant science · 2022Review
- In Vitro Tissue Culture inInternational journal of molecular sciences · 2020Review
- A CRISPR/Cas9-Based Mutagenesis Protocol forFrontiers in plant science · 2020Article
- Review
- Spatial Distribution of Selected Chemical Cell Wall Components in the Embryogenic Callus of Brachypodium distachyon.PloS one · 2016Article
- Reduced susceptibility to Fusarium head blight in Brachypodium distachyon through priming with the Fusarium mycotoxin deoxynivalenol.Molecular plant pathology · 2015Article
- O-GlcNAc-mediated interaction between VER2 and TaGRP2 elicits TaVRN1 mRNA accumulation during vernalization in winter wheat.Nature communications · 2014Article
- Parallel analysis of RNA ends enhances global investigation of microRNAs and target RNAs of Brachypodium distachyon.Genome biology · 2013Article
- Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species.BMC plant biology · 2012Article
- Plant regeneration from immature inflorescence derived callus cultures of salt tolerant kallar grass (Leptochloa fusca L.).Physiology and molecular biology of plants : an international journal of functional plant biology · 2012Article
- Diversity of morphology and function in arbuscular mycorrhizal symbioses in Brachypodium distachyon.Planta · 2012Article
- An efficient method for transient gene expression in monocots applied to modify the Brachypodium distachyon cell wall.Annals of botany · 2012Article
- Cell wall composition throughout development for the model grass Brachypodium distachyon.Frontiers in plant science · 2012Article
- Generation and characterization of the Western Regional Research Center Brachypodium T-DNA insertional mutant collection.PloS one · 2012Article
- Investigations of barley stripe mosaic virus as a gene silencing vector in barley roots and in Brachypodium distachyon and oat.Plant methods · 2010Article
- A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21.Nature protocols · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A fast and efficient microprojectile bombardment-mediated transformation protocol is reported for the grass species Brachypodium distachyon, a proposed alternative model plant to Oryza sativa for functional genomics in grasses. Embryogenic calli derived from immature embryos were transformed by a construct containing the uidA (coding for beta-glucuronidase) and bar (coding for phosphinothricin acetyl transferase) genes, and bialaphos, a non-selective herbicide, was used as the selection agent throughout all phases of the tissue culture. Average transformation efficiencies of 5.3% were achieved, and for single bombardments transformation efficiencies of up to 14% were observed. The time frame from the bombardment of embryogenic callus to the harvesting of transgenic T1 seeds was 29 weeks and 25 weeks for the diploid and two tetraploid accessions used, respectively. Since the seed-to-seed life cycle is 19 weeks for the diploid and 15 weeks for the tetraploid accessions, our B. distachyon transformation system allows testing of both the T0 and the T1 generation as well as production of T2 seeds within 1 year.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.