ArticleBiophysical journal2005
Mitochondrial Ca2+ dynamics reveals limited intramitochondrial Ca2+ diffusion.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
- Diffusive spreading across dynamic mitochondrial network architectures.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Mitochondrial Calcium Uniporter Regulates ITAM-Dependent Platelet Activation.Circulation research · 2025Article
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- Mitochondrial Calcium Waves by Electrical Stimulation in Cultured Hippocampal Neurons.Molecular neurobiology · 2024Article
- Spinning Disk Confocal Microscopy for Optimized and Quantified Live Imaging of 3D Mitochondrial Network.International journal of molecular sciences · 2024Article
- Stress-dependent macromolecular crowding in the mitochondrial matrix.The EMBO journal · 2023Article
- MICU1 controls spatial membrane potential gradients and guides CaCommunications biology · 2022Article
- MICU1-dependent mitochondrial calcium uptake regulates lung alveolar type 2 cell plasticity and lung regeneration.JCI insight · 2022Article
- Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing.Biology open · 2020Article
- CaBiophysical journal · 2020Article
- Resistive flow sensing of vital mitochondria with nanoelectrodes.Mitochondrion · 2017Article
- Age decreases mitochondrial motility and increases mitochondrial size in vascular smooth muscle.The Journal of physiology · 2016Article
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- Flicker-assisted localization microscopy reveals altered mitochondrial architecture in hypertension.Scientific reports · 2015Article
- Ca2+-mediated mitochondrial reactive oxygen species metabolism augments Wnt/β-catenin pathway activation to facilitate cell differentiation.The Journal of biological chemistry · 2014Article
- Mitochondrial calcium handling within the interstitial cells of Cajal.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
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- No consistent bioenergetic defects in presynaptic nerve terminals isolated from mouse models of Alzheimer's disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Forward operation of adenine nucleotide translocase during F0F1-ATPase reversal: critical role of matrix substrate-level phosphorylation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Real-time visualization of cytoplasmic calpain activation and calcium deregulation in acute glutamate excitotoxicity.Journal of neurochemistry · 2009Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To reveal heterogeneity of mitochondrial function on the single-mitochondrion level we have studied the spatiotemporal dynamics of the mitochondrial Ca2+ signaling and the mitochondrial membrane potential using wide-field fluorescence imaging and digital image processing techniques. Here we demonstrate first-time discrete sites--intramitochondrial hotspots--of Ca2+ uptake after Ca2+ release from intracellular stores, and spreading of Ca2+ rise within the mitochondria. The phenomenon was characterized by comparison of observations in intact cells stimulated by ATP and in plasma membrane permeabilized or in ionophore-treated cells exposed to elevated buffer [Ca2+]. The findings indicate that Ca2+ diffuses laterally within the mitochondria, and that the diffusion is limited for shorter segments of the mitochondrial network. These observations were supported by mathematical simulation of buffered diffusion. The mitochondrial membrane potential was investigated using the potentiometric dye TMRM. Irradiation-induced fluctuations (flickering) of TMRM fluorescence showed synchronicity over large regions of the mitochondrial network, indicating that certain parts of this network form electrical syncytia. The spatial extension of these syncytia was decreased by 2-aminoethoxydiphenyl borate (2-APB) or by propranolol (blockers of nonclassical mitochondrial permeabilities). Our data suggest that mitochondria form syncytia of electrical conductance whereas the passage of Ca2+ is restricted to the individual organelle.
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