ArticleBiophysical journal2005
Mechanical properties of Xenopus egg cytoplasmic extracts.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 101 citations in OpenAlex.
- Reconstitution of actomyosin networks in cell-sized liposomes dissects distinct mechanisms of membrane blebbing and symmetry breaking.Science advances · 2026Article
- Energy-precision trade-off in mitotic oscillators revealed by ATP modulation in artificial cells.bioRxiv : the preprint server for biology · 2026Article
- An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extracts.Journal of cell science · 2025Article
- Episodic transport of protein aggregates achieves a positive size selectivity in aggresome formation.Nature communications · 2025Article
- Reconstitution of actomyosin networks in cell-sized liposomes reveals distinct mechanical roles of cytoskeletal organization in membrane shape remodeling.bioRxiv : the preprint server for biology · 2025Article
- Size- and position-dependent cytoplasm viscoelasticity through hydrodynamic interactions with the cell surface.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- YAP Inactivation by Soft Mechanotransduction Relieves MAFG for Tumor Cell Dedifferentiation.Research (Washington, D.C.) · 2023Article
- Unveiling the physics underlying symmetry breaking of the actin cytoskeleton: An artificial cell-based approach.Biophysics and physicobiology · 2023Article
- Cytoplasmic organization promotes protein diffusion in Xenopus extracts.Nature communications · 2022Article
- Contribution of cytoplasm viscoelastic properties to mitotic spindle positioning.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Single-Particle Tracking Reveals Anti-Persistent Subdiffusion in Cell Extracts.Entropy (Basel, Switzerland) · 2021Article
- Article
- The Poisson Ratio of the Cellular Actin Cortex Is Frequency Dependent.Biophysical journal · 2020Article
- Disassembly of Actin and Keratin Networks by Aurora B Kinase at the Midplane of Cleaving Xenopus laevis Eggs.Current biology : CB · 2019Article
- Tissue cell differentiation and multicellular evolution via cytoskeletal stiffening in mechanically stressed microenvironments.Acta mechanica Sinica = Li xue xue bao · 2019Article
- Can diffusion-weighted imaging predict tumor grade and expression of Ki-67 in breast cancer? A multicenter analysis.Breast cancer research : BCR · 2018Article
- Universal glass-forming behavior of in vitro and living cytoplasm.Scientific reports · 2017Article
- Associations between apparent diffusion coefficient (ADC) and KI 67 in different tumors: a meta-analysis. Part 1: ADCOncotarget · 2017Article
- High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications.Cytoskeleton (Hoboken, N.J.) · 2017Article
- Microfluidic analysis of oocyte and embryo biomechanical properties to improve outcomes in assisted reproductive technologies.Molecular human reproduction · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cytoplasmic extracts prepared from Xenopus laevis eggs are used for the reconstitution of a wide range of processes in cell biology, and offer a unique environment in which to investigate the role of cytoplasmic mechanics without the complication of preorganized cellular structures. As a step toward understanding the mechanical properties of this system, we have characterized the rheology of crude interphase extracts. At macroscopic length scales, the extract forms a soft viscoelastic solid. Using a conventional mechanical rheometer, we measure the elastic modulus to be in the range of 2-10 Pa, and loss modulus in the range of 0.5-5 Pa. Using pharmacological and immunological disruption methods, we establish that actin filaments and microtubules cooperate to give mechanical strength, whereas the intermediate filament cytokeratin does not contribute to viscoelasticity. At microscopic length scales smaller than the average network mesh size, the response is predominantly viscous. We use multiple particle tracking methods to measure the thermal fluctuations of 1 microm embedded tracer particles, and measure the viscosity to be approximately 20 mPa-s. We explore the impact of rheology on actin-dependent cytoplasmic contraction, and find that although microtubules modulate contractile forces in vitro, their interactions are not purely mechanical.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.