ArticleInfection and immunity2004
The hyphal and yeast forms of Candida albicans bind the complement regulator C4b-binding protein.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 116 citations in OpenAlex.
- Binding of complement inhibitor C4b-binding protein to a highly virulent Streptococcus pyogenes M1 strain is mediated by protein H and enhances adhesion to and invasion of endothelial cells.The Journal of biological chemistry · 2013Trial
- Review
- ControllingInfection and immunity · 2024Review
- Diverse Functions of C4b-Binding Protein in Health and Disease.Journal of immunology (Baltimore, Md. : 1950) · 2023Review
- Antifungal Potential of Some Herb Decoctions and Essential Oils onHealthcare (Basel, Switzerland) · 2022Article
- Detection ofMicromachines · 2021Article
- Interactions Between PathogenicFrontiers in cellular and infection microbiology · 2021Review
- Complement Evasion Strategies of Human Pathogenic Bacteria.Indian journal of microbiology · 2020Review
- Moonlighting Proteins at the Candidal Cell Surface.Microorganisms · 2020Review
- Review
- Antibacterial Fusion Proteins EnhanceFrontiers in immunology · 2020Article
- Podocytes Produce and Secrete Functional Complement C3 and Complement Factor H.Frontiers in immunology · 2020Article
- Mechanisms of PathogenicFrontiers in cellular and infection microbiology · 2020Review
- C4BP-IgM protein as a therapeutic approach to treat Neisseria gonorrhoeae infections.JCI insight · 2019Article
- Antivirulence and avirulence genes in human pathogenic fungi.Virulence · 2019Review
- Candida albicans Elicits Pro-Inflammatory Differential Gene Expression in Intestinal Peyer's Patches.Mycopathologia · 2019Article
- Immunopathology of Recurrent Vulvovaginal Infections: New Aspects and Research Directions.Frontiers in immunology · 2019Review
- The Pneumococcal Surface Proteins PspA and PspC Sequester Host C4-Binding Protein To Inactivate Complement C4b on the Bacterial Surface.Infection and immunity · 2019Article
- Article
- The Expanding Landscape of Moonlighting Proteins in Yeasts.Microbiology and molecular biology reviews : MMBR · 2016Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candida albicans, an important pathogenic yeast, activates all three pathways of the complement system. To understand how this yeast evades the effects of the activated system, we have analyzed the binding of the classical pathway inhibitor C4b-binding protein (C4BP) by C. albicans. Purified native as well as recombinant C4BP bound dose dependently to the yeast and hyphal forms, as shown by multiple methods, such as confocal microscopy, flow cytometry, a novel enzyme-linked immunosorbent assay, absorption from human serum, and direct binding assays with purified proteins. A prominent binding site was identified at the tip of the germ tube, a structure that is considered important for tissue penetration and pathogenesis. The binding site in C4BP was localized to the two N-terminal complement control protein domains by using recombinant deletion constructs and site-specific monoclonal antibodies. As the alternative pathway inhibitors factor H and FHL-1 also bind to C. albicans, the binding of all three plasma proteins was compared. Simultaneous binding of the classical regulator C4BP and the alternative pathway regulator factor H was demonstrated by confocal microscopy. In addition, FHL-1 competed for binding with C4BP, suggesting that these two related complement regulators bind to the same structures on the yeast surface. The surface-attached C4BP maintains its complement regulatory activities and inactivates C4b. The surface-attached human C4BP serves multiple functions relevant for immune evasion and likely pathogenicity. It inhibits complement activation at the yeast surface and, in addition, mediates adhesion of C. albicans to host endothelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.