ArticleThe Biochemical journal2005
Manganese toxicity and Saccharomyces cerevisiae Mam3p, a member of the ACDP (ancient conserved domain protein) family.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Molecular bases of circadian magnesium rhythms across eukaryotes.FEBS letters · 2026Article
- Enabling malic acid production from corn-stover hydrolysate in Lipomyces starkeyi via metabolic engineering and bioprocess optimization.Microbial cell factories · 2025Article
- Conserved Genes in Highly Regenerative Metazoans Are Associated with Planarian Regeneration.Genome biology and evolution · 2024Article
- Thiolutin has complex effects in vivo but is a direct inhibitor of RNA polymerase II in vitro.Nucleic acids research · 2024Article
- A tonoplast-localized magnesium transporter is crucial for stomatal opening in Arabidopsis under high MgThe New phytologist · 2022Article
- Conserved mechanism for vacuolar magnesium sequestration in yeast and plant cells.Nature plants · 2022Article
- Fungal-Metal Interactions: A Review of Toxicity and Homeostasis.Journal of fungi (Basel, Switzerland) · 2021Review
- Novel Aspects of Renal Magnesium Homeostasis.Frontiers in pediatrics · 2018Review
- A Medicago truncatula Cystathionine-β-Synthase-like Domain-Containing Protein Is Required for Rhizobial Infection and Symbiotic Nitrogen Fixation.Plant physiology · 2016Article
- Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca2+ in Hansenula polymorpha.PloS one · 2015Article
- Nucleotide binding triggers a conformational change of the CBS module of the magnesium transporter CNNM2 from a twisted towards a flat structure.The Biochemical journal · 2014Article
- Identification and lateral membrane localization of cyclin M3, likely to be involved in renal Mg2+ handling in seawater fish.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Article
- Purification, crystallization and preliminary crystallographic analysis of the CBS-domain pair of cyclin M2 (CNNM2).Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- The role of the Parkinson's disease gene PARK9 in essential cellular pathways and the manganese homeostasis network in yeast.PloS one · 2012Article
- Purification, crystallization and preliminary crystallographic analysis of the CBS pair of the human metal transporter CNNM4.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- A genome-wide immunodetection screen in S. cerevisiae uncovers novel genes involved in lysosomal vacuole function and morphology.PloS one · 2011Article
- The effect of phosphate accumulation on metal ion homeostasis in Saccharomyces cerevisiae.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2010Article
- Manganese homeostasis in Saccharomyces cerevisiae.Chemical reviews · 2009Review
- Down-regulation of a manganese transporter in the face of metal toxicity.Molecular biology of the cell · 2009Article
- Mutations in CNNM4 cause recessive cone-rod dystrophy with amelogenesis imperfecta.American journal of human genetics · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Manganese is an essential, but potentially toxic, trace metal in biological systems. Overexposure to manganese is known to cause neurological deficits in humans, but the pathways that lead to manganese toxicity are largely unknown. We have employed the bakers' yeast Saccharomyces cerevisiae as a model system to identify genes that contribute to manganese-related damage. In a genetic screen for yeast manganese-resistance mutants, we identified S. cerevisiae MAM3 as a gene which, when deleted, would increase cellular tolerance to toxic levels of manganese and also increased the cell's resistance towards cobalt and zinc. By sequence analysis, Mam3p shares strong similarity with the mammalian ACDP (ancient conserved domain protein) family of polypeptides. Mutations in human ACDP1 have been associated with urofacial (Ochoa) syndrome. However, the functions of eukaryotic ACDPs remain unknown. We show here that S. cerevisiae MAM3 encodes an integral membrane protein of the yeast vacuole whose expression levels directly correlate with the degree of manganese toxicity. Surprisingly, Mam3p contributes to manganese toxicity without any obvious changes in vacuolar accumulation of metals. Furthermore, through genetic epistasis studies, we demonstrate that MAM3 operates independently of the well-established manganese-trafficking pathways in yeast, involving the manganese transporters Pmr1p, Smf2p and Pho84p. This is the first report of a eukaryotic ACDP family protein involved in metal homoeostasis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.