ArticleRNA (New York, N.Y.)2004
Two distinct Staufen isoforms in Xenopus are vegetally localized during oogenesis.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 60 citations in OpenAlex.
- Germ plasm dynamics during oogenesis and early embryonic development in Xenopus and zebrafish.Molecular reproduction and development · 2024Review
- Remnants of the Balbiani body are required for formation of RNA transport granules iniScience · 2022Article
- High Level of Staufen1 Expression Confers Longer Recurrence Free Survival to Non-Small Cell Lung Cancer Patients by Promoting THBS1 mRNA Degradation.International journal of molecular sciences · 2021Article
- L-bodies are RNA-protein condensates driving RNA localization inMolecular biology of the cell · 2021Article
- An Emerging Role for Post-translational Modifications in Regulating RNP Condensates in the Germ Line.Frontiers in molecular biosciences · 2021Review
- A multipronged approach to understanding the form and function of hStaufen protein.RNA (New York, N.Y.) · 2020Article
- The M-phase specific hyperphosphorylation of Staufen2 involved the cyclin-dependent kinase CDK1.BMC cell biology · 2017Article
- Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1.Molecular & cellular proteomics : MCP · 2016Article
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- Global analysis of asymmetric RNA enrichment in oocytes reveals low conservation between closely related Xenopus species.Molecular biology of the cell · 2015Article
- The multifunctional Staufen proteins: conserved roles from neurogenesis to synaptic plasticity.Trends in neurosciences · 2014Review
- c-Jun N-terminal kinase phosphorylation of heterogeneous nuclear ribonucleoprotein K regulates vertebrate axon outgrowth via a posttranscriptional mechanism.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination.RNA (New York, N.Y.) · 2013Article
- Staufen1 dimerizes through a conserved motif and a degenerate dsRNA-binding domain to promote mRNA decay.Nature structural & molecular biology · 2013Article
- Staufen2 functions in Staufen1-mediated mRNA decay by binding to itself and its paralog and promoting UPF1 helicase but not ATPase activity.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Bacterial expression strategies for several Sus scrofa diacylglycerol kinase alpha constructs: solubility challenges.Scientific reports · 2013Article
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- Xenopus staufen2 is required for anterior endodermal organ formation.Genesis (New York, N.Y. : 2000) · 2012Article
- Control of cytoplasmic mRNA localization.Cellular and molecular life sciences : CMLS · 2012Review
- Systematic and single cell analysis of Xenopus Piwi-interacting RNAs and Xiwi.The EMBO journal · 2009Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Localization of mRNA is an important way of generating early asymmetries in the developing embryo. In Drosophila, Staufen is intimately involved in the localization of maternally inherited mRNAs critical for cell fate determination in the embryo. We show that double-stranded RNA-binding Staufen proteins are present in the oocytes of a vertebrate, Xenopus, and are localized to the vegetal cytoplasm, a region where important mRNAs including VegT and Vg1 mRNA become localized. We identified two Staufen isoforms named XStau1 and XStau2, where XStau1 was found to be the principal Staufen protein in oocytes, eggs, and embryos, the levels of both proteins peaking during mid-oogenesis. In adults, Xenopus Staufens are principally expressed in ovary and testis. XStau1 was detectable throughout the oocyte cytoplasm by immunofluorescence and was concentrated in the vegetal cortical region from stage II onward. It showed partial codistribution with subcortical endoplasmic reticulum (ER), raising the possibility that Staufen may anchor mRNAs to specific ER-rich domains. We further showed that XStau proteins are transiently phosphorylated by the MAPK pathway during meiotic maturation, a period during which RNAs such as Vg1 RNA are released from their tight localization at the vegetal cortex. These findings provide evidence that Staufen proteins are involved in targeting and/or anchoring of maternal determinants to the vegetal cortex of the oocyte in Xenopus. The Xenopus oocyte should thus provide a valuable system to dissect the role of Staufen proteins in RNA localization and vertebrate development.
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